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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Telomere and 45S rDNA sequences are structurally linked on the chromosomes in Chrysanthemum segetum L
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Telomere and 45S rDNA sequences are structurally linked on the chromosomes in Chrysanthemum segetum L

机译：端粒和45S rDNA序列在菊花Segetum L的染色体上结构连接

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摘要

Some reports have shown that nucleolar organizer regions are located at the telomeric region and have a structural connection with telomeres at the cellular level in many organisms. In this study, we found that all 45S ribosomal DNA (rDNA) signals were located at telomeric regions on the chromosomes in Chrysanthemum segetum L., and the 45S rDNA showed distinct signal patterns on different metaphase chromosome spreads. The bicolor fluorescence in situ hybridization experiment on the extended fibers revealed that telomere repeats were structurally connected with or interspersed into rDNA sequences. The close cytological structure relation between rDNA and telomere sequences led us to use PCR with combinations of the telomere primer and the rDNA primer to obtain some fragments, which were flanked by different rDNA and telomere primer sequences. One representative clone CHS2 contains closely connected rDNA and telomere sequences, suggesting that the telomere sequence invaded into the conserved rDNA sequence. In addition, the sequences of some PCR clones were flanked by the single telomeric primer sequence or the rDNA primer sequence. These results suggested that homologous recombination occurred between tandem repeat units of rDNA sequences or telomere repeats at the chromosome terminus.
机译：一些报告表明，在许多生物中，核仁组织区位于端粒区域，并且在细胞水平上与端粒具有结构联系。在这项研究中，我们发现所有45S核糖体DNA（rDNA）信号都位于菊花Segetum L.染色体的端粒区域，而45S rDNA在不同的中期染色体扩展上显示出不同的信号模式。在延伸纤维上进行的双色荧光原位杂交实验表明，端粒重复序列与rDNA序列结构连接或散布在其中。 rDNA和端粒序列之间紧密的细胞学结构联系使我们使用结合端粒引物和rDNA引物的PCR来获得一些片段，这些片段的侧翼是不同的rDNA和端粒引物序列。一个代表性的克隆CHS2包含紧密相连的rDNA和端粒序列，这表明端粒序列侵入了保守的rDNA序列。另外，一些PCR克隆的序列侧接单个端粒引物序列或rDNA引物序列。这些结果表明在染色体末端的rDNA序列的串联重复单元或端粒重复序列之间发生了同源重组。

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