...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Involvement of phospholipase D-related signal transduction in chemical-induced programmed cell death in tomato cell cultures
【24h】

Involvement of phospholipase D-related signal transduction in chemical-induced programmed cell death in tomato cell cultures

机译：磷脂酶D相关信号转导参与番茄细胞培养物中化学诱导的程序性细胞死亡

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phospholipase D (PLD) and its product phosphatidic acid (PA) are incorporated in a complex metabolic network in which the individual PLD isoforms are suggested to regulate specific developmental and stress responses, including plant programmed cell death (PCD). Despite the accumulating knowledge, the mechanisms through which PLD/PA operate during PCD are still poorly understood. In this work, the role of PLDα1 in PCD and the associated caspase-like proteolysis, ethylene and hydrogen peroxide (H_2O_2) synthesis in tomato suspension cells was studied. Wild-type (WT) and PLDα1-silenced cell lines were exposed to the cell death-inducing chemicals camptothecin (CPT), fumonisin B1 (FB1) and CdSO_4. A range of caspase inhibitors effectively suppressed CPT-induced PCD in WT cells, but failed to alleviate cell death in PLDα1-deficient cells. Compared to WT, in CPT-treated PLDα1 mutant cells, reduced cell death and decreased production of H_2O_2 were observed. Application of ethylene significantly enhanced CPT-induced cell death both in WT and PLDα1 mutants. Treatments with the PA derivative lyso-phosphatidic acid and mastoparan (agonist of PLD/PLC signalling downstream of G proteins) caused severe cell death. Inhibitors, specific to PLD and PLC, remarkably decreased the chemical-induced cell death. Taken together with our previous findings, the results suggest that PLDα1 contributes to caspase-like-dependent cell death possibly communicated through PA, reactive oxygen species and ethylene. The dead cells expressed morphological features of PCD such as protoplast shrinkage and nucleus compaction. The presented findings reveal novel elements of PLD/PA-mediated cell death response and suggest that PLDα1 is an important factor in chemical-induced PCD signal transduction.
机译：磷脂酶D（PLD）及其产物磷脂酸（PA）被掺入复杂的代谢网络中，在该网络中建议单个PLD亚型调节特定的发育和胁迫反应，包括植物程序性细胞死亡（PCD）。尽管有积累的知识，但对PCD期间PLD / PA运行的机制仍然知之甚少。在这项工作中，研究了PLDα1在PCD中的作用以及相关的胱天蛋白酶样蛋白水解，乙烯和过氧化氢（H_2O_2）在番茄悬浮细胞中的合成。将野生型（WT）和PLDα1沉默的细胞系暴露于诱导细胞死亡的化学物质喜树碱（CPT），伏马菌素B1（FB1）和CdSO_4。一系列半胱天冬酶抑制剂可有效抑制WT细胞中CPT诱导的PCD，但未能减轻PLDα1缺陷细胞的细胞死亡。与WT相比，在CPT处理的PLDα1突变细胞中，观察到细胞死亡减少和H_2O_2产生减少。乙烯的应用显着增强了WT和PLDα1突变体中CPT诱导的细胞死亡。用PA衍生物溶血磷脂酸和乳脂素（G蛋白下游PLD / PLC信号传导的激动剂）进行的治疗会导致严重的细胞死亡。 PLD和PLC特有的抑制剂可显着降低化学诱导的细胞死亡。结合我们以前的发现，结果表明PLDα1可能通过PA，活性氧和乙烯与半胱天冬酶样依赖性细胞死亡有关。死细胞表达PCD的形态特征，例如原生质体收缩和细胞核紧实。提出的发现揭示了PLD / PA介导的细胞死亡反应的新元素，并表明PLDα1是化学诱导的PCD信号转导的重要因素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号