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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Isolation and characterization of the ejectisomes of the prasinophyte Pyramimonas grossii
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Isolation and characterization of the ejectisomes of the prasinophyte Pyramimonas grossii

机译：藻类植物Pyramimonas grossii弹射体的分离与鉴定

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摘要

For the first time, ejectisome-enriched fractions were isolated from the marine prasinophyte Pyramimonas grossii. Transmission electron microscopy revealed that most of the ejectisomes were discharged and formed long, spirally twisted filaments. Some ejectisomes were still fully or partly furled. Discharged ejectisomes measured up to 26 μm in length and 200 nm in width; those still furled measured up to 900 nm in width and 1,000 nm in length. Particle periodicities of approximately 4.2 and 5.8 nm could be measured from freeze-fractured filaments. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a protein banding pattern, dominated by polypeptides of 16-20 kDa. These polypeptides were not glycosylated and did not cross-react with antisera directed against recombinant R-body polypeptides of Caedibacter taeniospiralis or directed against reconstituted cryptophycean ejectisomes.
机译：第一次，从海洋类植物藻类Pyramimonas grossii中分离出富含喷射体的馏分。透射电子显微镜显示，大多数喷射异构体被释放并形成长螺旋扭曲的细丝。一些喷射异构体仍然全部或部分卷曲。放电射出物的尺寸最长可达26μm，宽度可达200​​ nm;仍在堆焊的样品的宽度可达900 nm，长度可达1,000 nm。从冷冻断裂的长丝可以测得约4.2和5.8nm的颗粒周期性。十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示出蛋白条带模式，主要由16-20 kDa的多肽组成。这些多肽没有被糖基化，并且不会与针对牛带弯曲杆菌的重组R-体多肽的抗血清或针对重构的隐藻菌射出异构体的抗血清发生交叉反应。

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