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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection
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Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection

机译：葡萄网膜葡萄球菌感染葡萄的PR10蛋白基因的亚细胞定位和功能分析

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摘要

Downy mildew, caused by the oomycete Plasmopara viticola, is a serious fungal disease in the cultivated European grapevines (Vitis vinifera L.). The class 10 of pathogenesis-related (PR) genes in grapevine leaves was reported to be accumulated at mRNA level in response to P. viticola infection. To elucidate the functional roles of PR10 genes during plant-pathogen interactions, a PR10 gene from a fungal-resistant accession of Chinese wild Vitis pseudoreticulata (designated VpPR10. 2) was isolated and showed high homology to PR10. 2 from susceptible V. vinifera (designated VvPR10. 2). Comparative analysis displayed that there were significant differences in the patterns of gene expression between the PR10 genes from the two host species. VpPR10. 2 was induced with high level in leaves infected by P. viticola, while VvPR10. 2 showed a low response to this inoculation. Recombinant VpPR10. 2 protein showed DNase activity against host genomic DNA and RNase activity against yeast total RNA in vitro. Meanwhile, recombinant VpPR10. 2 protein inhibited the growth of tobacco fungus Alternaria alternata and over-expression of VpPR10. 2 in susceptible V. vinifera enhanced the host resistance to P. viticola. The results from subcellular localization analysis showed that VpPR10. 2 proteins were distributed dynamically inside or outside of host cell. Moreover, they were found in haustorium of P. viticola and nucleus of host cell which was associated with a nucleus collapse at 10 days post-inoculation. Taken together, these results suggested that VpPR10. 2 might play an important role in host plant defense against P. viticola infection.
机译：由卵菌疟原虫（Plasmopara viticola）引起的霜霉病是欧洲栽培葡萄（Vitis vinifera L.）中的一种严重的真菌病。据报道，葡萄叶中的发病机理相关（PR）基因的第10类在小葡萄球菌感染后以mRNA水平积累。为了阐明PR10基因在植物与病原体相互作用中的功能作用，从中国野生葡萄假单胞菌（命名为VpPR10。2）的抗真菌材料中分离出一个PR10基因，并显示出与PR10的高度同源性。 2来自易感的V. vinifera（命名为VvPR10.2）。对比分析显示，来自两种宿主物种的PR10基因之间的基因表达模式存在显着差异。 VpPR10。在被葡萄球菌感染的叶片中高水平诱导2，而VvPR10。图2显示了对该接种的低响应。重组VpPR10。 2种蛋白在体外显示出针对宿主基因组DNA的DNase活性和针对酵母总RNA的RNase活性。同时，重组VpPR10。 2蛋白抑制烟草真菌Alternaria alternata的生长和VpPR10的过表达。易感的V. vinifera中的2增强了宿主对P. viticola的抗性。亚细胞定位分析的结果显示为VpPR10。 2种蛋白质在宿主细胞内部或外部动态分布。此外，它们被发现在葡萄球菌的败血症和宿主细胞的核中，这些与接种后10天的核塌陷有关。综上，这些结果表明是VpPR10。 2可能在寄主植物防御葡萄小球菌感染中起重要作用。

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