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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >IDENTIFICATION AND LOCALIZATION OF CALRETICULIN IN PLANT CELLS
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IDENTIFICATION AND LOCALIZATION OF CALRETICULIN IN PLANT CELLS

机译:植物细胞中钙网蛋白的鉴定和定位

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In the present study we have investigated the presence and distribution of calreticulin in plant protoplasts. Calreticulin was purified from plant homogenates using a selective ammonium sulfate precipitation procedure developed for the purification of mammalian calreticulins and shown to bind calcium in (45)-Ca2+ overlay assays. The protein was localized to plant cell endoplasmic reticulum by the indirect immunofluorescence staining of protoplasts with anti-calreticulin antibodies. No calreticulin was observed within large vacuoles. We conclude that calreticulin is present in the endoplasmic reticulum of plant cells, when, by analogy to the mammalian endoplasmic reticulum, it may play a major role in Ca2+ binding and storage. [References: 36]
机译:在本研究中,我们已经研究了钙网蛋白在植物原生质体中的存在和分布。钙网蛋白是使用选择性硫酸铵沉淀法从植物匀浆中纯化的,该沉淀法是为纯化哺乳动物钙网蛋白而开发的,并在(45)-Ca2 +重叠分析中显示出与钙的结合。通过用抗钙网蛋白抗体对原生质体进行间接免疫荧光染色,将蛋白质定位于植物细胞内质网。大液泡内未观察到钙网蛋白。我们得出结论,钙网蛋白存在于植物细胞的内质网中,与哺乳动物内质网类似,它可能在Ca2 +的结合和储存中起主要作用。 [参考:36]

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