FLUORESCENT LABELLING OF NASCENT RNA REVEALS NUCLEAR TRANSCRIPTION DOMAINS THROUGHOUT PLANT CELL NUCLEI

Straatman KR.; Schul W.; Schel JHN.; Trompetter CM.

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FLUORESCENT LABELLING OF NASCENT RNA REVEALS NUCLEAR TRANSCRIPTION DOMAINS THROUGHOUT PLANT CELL NUCLEI

机译：贯穿整个植物细胞核的NASCENT RNA揭示核转录域的荧光标记

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Transcription and RNA processing are main functions of the nucleus. These processes are found localized in specific nuclear domains. We have investigated the presence of nuclear transcription domains in plants by incorporating 5-bromouridine 5'-triphosphate in nascent RNA in isolated nuclei of Brassica napus. Bromo-uridine labelled RNA was visualized by a FITC-labelled biotin-avidin system in combination with confocal laser scanning microscopy. Labelled domains were found throughout the nucleus, in some cases including the nucleolus. This shows that the distribution of transcription sites in plant nuclei is similar to that of mammalian nuclei and that the same labelling procedures can be used. [References: 21]

机译：转录和RNA加工是细胞核的主要功能。发现这些过程位于特定的核域中。我们通过在甘蓝型油菜的分离核中将新生的RNA中掺入5-溴尿苷5'-三磷酸来研究植物中核转录结构域的存在。溴尿苷标记的RNA通过FITC标记的生物素-亲和素系统与共焦激光扫描显微镜相结合可视化。在整个细胞核中发现标记的结构域，在某些情况下包括核仁。这表明植物核中转录位点的分布与哺乳动物核相似，并且可以使用相同的标记程序。 [参考：21]

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《Protoplasma: An International Journal of Cell Biology》 |1996年第4期|共5页
作者
Straatman KR.; Schul W.; Schel JHN.; Trompetter CM.;
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正文语种 eng
中图分类细胞形态学;
关键词
Brassica napus; 5-bromouridine 5'-triphosphate incorporation; Confocal scanning laser microscopy; Immunolabelling; Isolated nuclei; Transcription; Brassica-napus l; Insitu hybridization; Ribosomal-rna; Visualization; Proteins; Pollen; Gene;

机译：甘蓝型油菜;5-溴尿苷5'-三磷酸酯的掺入;共聚焦激光扫描显微镜;免疫接种;分离的核;转录;甘蓝型油菜;原位杂交;核糖体-核糖核酸;可视化;蛋白质;花粉;基因;

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1. 多头绒泡菌间期细胞核中RNA的转录状况 [J] . 焦明大, 毕晓辉, 曾庆华, 植物学报（英文版） . 2001,第003期
2. FLUORESCENT LABELLING OF NASCENT RNA REVEALS NUCLEAR TRANSCRIPTION DOMAINS THROUGHOUT PLANT CELL NUCLEI [J] . Straatman KR., Schul W., Schel JHN., Protoplasma: An International Journal of Cell Biology . 1996,第3a4期

机译：贯穿整个植物细胞核的NASCENT RNA揭示核转录域的荧光标记
3. Halogenated nucleotide labeling of nascent RNAs reveals dynamic transcription in growing pig oocytes [J] . OqaniR.K., LeeM.G., DiaoY.F., Developmental dynamics: an official publication of the American Association of Anatomists . 2013,第1期

机译：新生RNA的卤代核苷酸标记揭示了正在生长的猪卵母细胞中的动态转录
4. Evolution of RLSB, a nuclear-encoded S1 domain RNA binding protein associated with post-transcriptional regulation of plastid-encoded rbc L mRNA in vascular plants [J] . Pradeep Yerramsetty, Matt Stata, Rebecca Siford, BMC Evolutionary Biology . 2016,第1期

机译：RLSB的进化，一种核编码的S1域RNA结合蛋白，与血管植物中质体编码的rbc L mRNA的转录后调控相关
5. Modeling of Fluorescent Protein-labeled Cell Populations to Analyze Transcriptional and Division Effects on Fluorescent Intensity Distributions [C] . Loveleena Bansal, Shreya Maiti, Arul Jayaraman, AIChE annual meeting . 2012

机译：荧光蛋白标记的细胞群体建模，以分析转录和分裂对荧光强度分布的影响
6. Advances in RNA Metabolic Labeling for Profiling Nascent Transcription [D] . Nainar, Sarah J. 2019

机译：RNA代谢标记的新陈代谢转录研究进展。
7. Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus [O] . 1993

机译：新生RNA的荧光标记揭示了RNA聚合酶II在遍布整个核的域中的转录
8. Fluorescent labelling of nascent RNA reveals nuclear transcription domains throughout plant cell nuclei [O] . Straatman, K.R., Trompetter, C.M., Schul, W., 1996

机译：新生RNA的荧光标记揭示了整个植物细胞核中的核转录域

FLUORESCENT LABELLING OF NASCENT RNA REVEALS NUCLEAR TRANSCRIPTION DOMAINS THROUGHOUT PLANT CELL NUCLEI

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