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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >ULTRASTRUCTURAL DETECTION OF SUCROSE SYNTHASE DISTRIBUTION IN DEVELOPING MAIZE LEAVES
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ULTRASTRUCTURAL DETECTION OF SUCROSE SYNTHASE DISTRIBUTION IN DEVELOPING MAIZE LEAVES

机译：玉米叶片发育过程中糖合酶分布的超微结构检测

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摘要

A developing maize leaf grows by the activity of a basal meristematic region and an adjacent elongating zone, resulting in a morphological and functional gradient along the leaf. We have used this system to detect the spatial and temporal expression of an enzyme, sucrose synthase, which plays a pivotal role in the sucrose import-export transition which occurs along a monocotyledon leaf. Immunogold labeling was used to detect the cellular and sub-cellular distribution of sucrose synthase (SS) at the electron microscopical level; the protein was visualized using a polyclonal antiserum on embedded tissue sections. Immunolabel was observed in the cytosol of dividing meristematic cells, expanding cells of the elongation zone, and in differentiating cells of young photosynthetic tissue. In fully differentiated leaf tissue, however, the protein was no longer immune-detectable in photosynthetic cells, but was present in the guard and subsidiary cells of stomata and in companion cells within the phloem tissue of vascular bundles. The tissue- and cell-specific localization of sucrose synthase changes along the growing leaf as a function of the developmental state and the associated need for sucrose import or export. [References: 19]
机译：发育中的玉米叶片通过基底分生组织区域和相邻的延伸区域的活动而生长，从而导致沿叶片的形态和功能梯度。我们已经使用该系统检测蔗糖合酶的空间和时间表达，该酶在沿单子叶植物叶片发生的蔗糖进出口过渡中起着关键作用。免疫金标记用于在电子显微镜下检测蔗糖合酶（SS）的细胞和亚细胞分布;使用嵌入式组织切片上的多克隆抗血清将蛋白质可视化。在分裂的分生组织细胞，延伸区域的扩展细胞以及年轻光合组织的分化细胞的胞质中观察到了免疫标记。然而，在完全分化的叶片组织中，该蛋白在光合细胞中不再能被免疫检测到，而是存在于气孔的保卫细胞和辅助细胞以及维管束韧皮部组织内的陪伴细胞中。蔗糖合酶的组织和细胞特异性定位沿着生长的叶子随发育状态以及蔗糖进出口的相关需求而变化。 [参考：19]

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