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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Inactivation of Ca~(2+)-induced ciliary reversal by high-salt extraction in the cilia of Paramecium
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Inactivation of Ca~(2+)-induced ciliary reversal by high-salt extraction in the cilia of Paramecium

机译：草履虫纤毛中高盐提取灭活Ca〜（2+）诱导的纤毛逆转

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摘要

Intracellular Ca~(2+) induces ciliary reversal and backward swimming in Paramecium. However, it is not known how the Ca~(2+) signal controls the motor machinery to induce ciliary reversal. We found that demembranated cilia on the ciliated cortical sheets from Paramecium caudatum lost the ability to undergo ciliary reversal after brief extraction with a solution containing 0.5 M KCl. KNO_3, which is similar to KCl with respect to chaotropic effect; it had the same effect as that of KCl on ciliary response. Cyclic AMP antagonizes Ca~(2+)-induced ciliary reversal. Limited trypsin digestion prevents endogenous A-kinase and cAMP-dependent phosphorylation of an outer arm dynein light chain and induces ciliary reversal. However, the trypsin digestion prior to the high-salt extraction did not affect the inhibition of Ca~(2+)-induced ciliary reversal caused by the high-salt extraction. Furthermore, during the course of the high-salt extraction, some axonemal proteins were extracted from ciliary axonemes, suggesting that they may be responsible for Ca~(2+)-induced ciliary reversal.
机译：细胞内Ca〜（2+）诱导草履虫发生睫状体逆转和向后游动。但是，尚不知道Ca〜（2+）信号如何控制电机引起睫状体逆转。我们发现用含0.5 M KCl的溶液短暂提取后，来自尾草履虫的纤毛皮质片上的去膜纤毛失去了进行纤毛逆转的能力。 KNO_3，就离液效应而言与KCl相似;它与KCl对睫状反应的作用相同。环状AMP拮抗Ca〜（2+）诱导的睫状肌逆转。有限的胰蛋白酶消化可防止外臂动力蛋白轻链的内源性A激酶和cAMP依赖性磷酸化，并诱导纤毛逆转。然而，高盐提取之前的胰蛋白酶消化并没有影响由高盐提取引起的Ca〜（2+）诱导的纤毛逆转的抑制。此外，在高盐提取过程中，从睫状轴突中提取了一些轴突蛋白，表明它们可能是由Ca〜（2+）引起的纤毛逆转的原因。

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