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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Cloning and molecular characterization of a putative bZIP transcription factor VvbZIP23 from Vitis vinifera
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Cloning and molecular characterization of a putative bZIP transcription factor VvbZIP23 from Vitis vinifera

机译：葡萄中推测的bZIP转录因子VvbZIP23的克隆和分子鉴定

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摘要

The proteins harboring bZIP domains comprise a large family and play key roles in many cellular processes, one of them being tolerance to biotic and abiotic stresses in plants. In the present study, we characterize a putative bZIP transcription factor from Vitis vinifera namely VvbZIP23. Our studies revealed that a GFP fusion of VvbZIP23 is localized in the nucleus showing VvbZIP23 codes for a nuclear localized protein. VvbZIP23 identified by in silico approaches from grapevine DNA databases available in the public domain NCBI is present in a single copy in the grapevine genome as shown by Southern blot analysis. Expression of VvbZIP23 is induced by a wide spectrum of abiotic stresses, including drought, salt, and cold. Exogenous application of signaling chemicals like abscisic acid, methyl viologen, salicylic acid, jasmonic acid, and ethephon also induced expression of VvbZIP23. This shows that VvbZIP23 is involved in regulating a number of stress responses in V. vinifera. The 5' proximal region of VvbZIP23 contains many cis-acting elements, which show induction of VvbZIP23 expression in multiple stress responses. Transcripts of VvbZIP23 were found in many parts of the grapevine plant with the highest expression detected in leaves. Further in silico analysis shows that the open reading frame of VvbZIP23 is 822 bp long and codes for a 273 amino acid long protein having a characteristic bZIP domain in its N-terminal end. Overexpression of VvbZIP23-GFP fusion protein in grapevine callus leads to enhanced transcript levels of genes, homologues of which are reported to be important in regulating many stress conditions.
机译：带有bZIP结构域的蛋白质包含一个大家族，在许多细胞过程中起着关键作用，其中之一是对植物中生物和非生物胁迫的耐受性。在本研究中，我们表征了来自葡萄的推定bZIP转录因子，即VvbZIP23。我们的研究表明，VvbZIP23的GFP融合物位于细胞核中，显示VvbZIP23编码核定位蛋白。如通过DNA印迹分析所示，通过计算机方法从公共领域NCBI中可获得的葡萄DNA数据库中鉴定的VvbZIP23存在于葡萄基因组中的单个拷贝中。 VvbZIP23的表达受多种非生物胁迫的诱导，包括干旱，盐分和寒冷。外源施用信号化学物质，如脱落酸，甲基紫精，水杨酸，茉莉酸和乙烯利，也会诱导VvbZIP23的表达。这表明VvbZIP23参与了V. vinifera的许多应激反应的调控。 VvbZIP23的5'近端区域包含许多顺式作用元件，这些元件在多种应激反应中均表现出VvbZIP23表达的诱导作用。在葡萄植物的许多部位发现了VvbZIP23的转录本，在叶片中检测到最高的表达。进一步的计算机分析表明，VvbZIP23的开放阅读框长822 bp，编码一个273个氨基酸长的蛋白，在其N端具有特征性bZIP结构域。 VvbZIP23-GFP融合蛋白在葡萄愈伤组织中的过表达导致基因的转录水平提高，据报道其同源物在调节许多胁迫条件中很重要。

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