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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Survival and ultrastructural features of peach palm (Bactris gasipaes, Kunth) somatic embryos submitted to cryopreservation through vitrification
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Survival and ultrastructural features of peach palm (Bactris gasipaes, Kunth) somatic embryos submitted to cryopreservation through vitrification

机译：通过玻璃化冷冻保存的桃棕榈（Bactris gasipaes，Kunth）体细胞胚的存活和超微结构特征

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摘要

Bactris gasipaes (Arecaceae), also known as peach palm, was domesticated by Amazonian Indians and is cultivated for its fruit and heart-of-palm, a vegetable grown in the tree's inner core. Currently, the conservation of this species relies on in situ conditions and field gene banks. Complementary conservation strategies, such as those based on in vitro techniques, are indicated in such cases. To establish an appropriate cryopreservation protocol, this study aimed to evaluate the ultrastructural features of B. gasipaes embryogenic cultures submitted to vitrification and subsequent cryogenic temperatures. Accordingly, somatic embryo clusters were submitted to Plant Vitrification Solution 3 (PVS3). In general, cells submitted to PVS3 had viable cell characteristics associated with apparently many mitochondria, prominent nucleus, and preserved cell walls. Cells not incubated in PVS3 did not survive after the cryogenic process in liquid nitrogen. The best incubation time for the vitrification technique was 240 min, resulting in a survival rate of 37 %. In these cases, several features were indicative of quite active cell metabolism, including intact nuclei and preserved cell walls, an apparently many of mitochondria and lipid bodies, and the presence of many starch granules and condensed chromatin. Moreover, ultrastructure analysis revealed that overall cellular structures had been preserved after cryogenic treatment, thus validating the use of vitrification in conjunction with cryopreservation of peach palm elite genotypes, as well as wild genotypes, which carry a rich pool of genes that must be conserved.
机译：Bactris gasipaes（Arecaceae），也称为桃棕榈，由亚马逊印第安人驯化，并以其果实和掌心（一种种植于树心的蔬菜）而种植。目前，该物种的保护依赖于原位条件和田间基因库。在这种情况下，应指出互补的保护策略，例如基于体外技术的策略。为了建立适当的冷冻保存方案，本研究旨在评估经受玻璃化和随后的低温处理的加倍芽孢杆菌胚发生培养物的超微结构特征。因此，将体细胞胚簇提交给植物玻璃化溶液3（PVS3）。通常，提交给PVS3的细胞具有与明显的线粒体，明显的细胞核和保存的细胞壁相关的活细胞特征。在液氮中进行低温处理后，未在PVS3中孵育的细胞无法存活。玻璃化技术的最佳孵育时间为240分钟，因此存活率为37％。在这些情况下，一些特征表明细胞代谢非常活跃，包括完整的细胞核和保留的细胞壁，明显的许多线粒体和脂质体，以及许多淀粉颗粒和浓缩的染色质。此外，超微结构分析显示，经过低温处理后，总体细胞结构得以保留，因此验证了玻璃化结合桃棕榈精英基因型以及野生基因型冷冻保存的应用，这些基因型携带着丰富的基因，必须进行保守。

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