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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Glutathione peroxidase expression and activity in barley root tip after short-term treatment with cadmium, hydrogen peroxide and t-butyl hydroperoxide
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Glutathione peroxidase expression and activity in barley root tip after short-term treatment with cadmium, hydrogen peroxide and t-butyl hydroperoxide

机译：镉，过氧化氢和叔丁基氢过氧化物短期处理后大麦根尖中谷胱甘肽过氧化物酶的表达和活性

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The purpose of this study was to analyse the alterations of glutathione peroxidase (GPX) expression and activity during the recovery period after a short-term treatment of barley root tip with cadmium (Cd) and hydrogen peroxide (H_2O_2). The transcript level of GPX increased as early as 1 h and GPX activity 3 h after short-term treatment independently of Cd concentration. In 15 μM Cd-treated roots, its expression reached a peak within 2 h and sustained until 3 h, after which it gradually declined. After 6 h of short-term Cd treatment, the activity of GPX was the highest in the 15-μM Cd-treated roots. At higher Cd concentrations, the activity of GPX was lower than in 15 μM Cd-treated roots, but still higher than in control roots. A considerable increase in H_2O_2 production was observed even after only 1 h of short-term exposure of roots to 30 and 60 μM Cd, while after 15 μM Cd exposure, its production increased 3 h after the treatment. Lipid peroxidation increased even 1 h after short-term treatment in a Cd concentration-dependent manner. A considerable decrease of GPX activity was observed after the exposure of roots to H_2O_2 or t-butyl hydroperoxide in a concentration-dependent manner despite that its expression increased even 1 h after short-term treatment. Presumable, under high acute Cd stress, rapid accumulation of H_2O_2 leads to the disturbance of basal metabolic processes affecting also GPX activity. In contrast, high GPX activity under moderate Cd stress maintains cell function despite the high rate of H_2O_2 metabolism in root tip.
机译：这项研究的目的是分析在镉（Cd）和过氧化氢（H_2O_2）短期处理大麦根尖后恢复期谷胱甘肽过氧化物酶（GPX）表达和活性的变化。短期治疗后，GPX的转录水平早于1 h升高，而GPX活性则在3 h升高，而与Cd浓度无关。在15μMCd处理的根中，其表达在2小时内达到峰值，并持续到3小时，此后逐渐下降。短期Cd处理6 h后，GPX的活性在15μMCd处理的根中最高。在较高的Cd浓度下，GPX的活性低于15μMCd处理的根，但仍高于对照根。即使仅将根部短期暴露于30和60μMCd中仅1 h，仍观察到H_2O_2产量的显着增加，而暴露15μMCd后，其处理后3 h的产量增加。短期治疗后1小时，脂质过氧化作用以Cd浓度依赖性方式增加。在根部以浓度依赖的方式将根暴露于H_2O_2或叔丁基氢过氧化物后，尽管其表达在短期处理后甚至增加了1小时，GPX活性仍显着下降。据推测，在高急性Cd胁迫下，H_2O_2的快速积累会导致基础代谢过程受到干扰，从而影响GPX活性。相反，尽管根尖中H_2O_2的代谢率很高，但在中等Cd胁迫下高GPX活性仍能维持细胞功能。

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