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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >GUS activity for miR165a/166b, REV, and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration
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GUS activity for miR165a/166b, REV, and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration

机译：miR165a / 166b，REV和WUS / CLV3在体外直接拟南芥芽再生中的GUS活性

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摘要

In Arabidopsis thaliana, the process of shoot regeneration in vitro requires the presence of specific miRNAs. We describe here the β-glucuronidase (GUS) expression domains for miR165a/166b, REV, and WUS/CLV3 during direct shoot regeneration. Increased GUS activity of miR166b and REV were first detected within the shoot apical meristem of explants, while no pmiR165a:GUS activity appeared there. The zone of pWUS:GUS activity covered the inner sides of developing protuberances, while that of pCLV3:GUS was more restricted. Once the primary shoot had emerged from the protuberance, pREV:GUS activity was turned on throughout the protuberance. pmiR165a:GUS activity was detected in a small number of protuberance surface cells, while pWUS:GUS activity was restricted to within a few cells beneath the protuberance surface. After the differentiation of leaf-like structures, GUS activity for miR165a and miR166b appeared largely on their abaxial surface, while pWUS:GUS activity was concentrated at the apex of the primary shoot, and no pCLV3:GUS activity was detectable. Following the formation of secondary shoots, pmiR165a:GUS activity was detected on their abaxial surface. GUS activity for miR166b, REV, and WUS/CLV3 were concentrated in the stem apical meristem. The observations suggested that each member of this set of genes might play a distinct role in both primary and secondary shoot regeneration.
机译：在拟南芥中，体外芽再生的过程需要存在特定的miRNA。我们在这里描述了miR165a / 166b，REV和WUS / CLV3在直接芽再生过程中的β-葡萄糖醛酸酶（GUS）表达域。首先在外植体的茎尖分生组织中检测到miR166b和REV的GUS活性增加，而在那里没有出现pmiR165a：GUS活性。 pWUS：GUS活动的区域覆盖了发育突起的内侧，而pCLV3：GUS的区域则受到更多限制。一旦从突起中出现初生芽，pREV：GUS活性就会在整个突起中打开。在少量突起表面细胞中检测到了pmiR165a：GUS活性，而pWUS：GUS活性被限制在突起表面以下的几个细胞内。在叶片状结构分化之后，miR165a和miR166b的GUS活性主要出现在它们的背面，而pWUS：GUS活性则集中在初生芽的顶端，而没有检测到pCLV3：GUS活性。在形成次生芽之后，在其背面上检​​测到pmiR165a：GUS活性。 miR166b，REV和WUS / CLV3的GUS活性集中在茎顶端分生组织中。观察结果表明，这组基因的每个成员可能在初级和次级芽再生中都起着不同的作用。

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