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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Kinetin induces cell death in root cortex cells of Vicia faba ssp. minor seedlings
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Kinetin induces cell death in root cortex cells of Vicia faba ssp. minor seedlings

机译：激动素诱导蚕豆（Vicia faba ssp）根皮层细胞死亡。小苗

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摘要

The double fluorescence staining with acridine orange and ethidium bromide (AO/EB) revealed that treatment of Vicia faba ssp. minor seedlings with kinetin-induced programmed cell death (PCD) in root cortex cells. Kinetin-induced cell death reflected by the morphological changes of nuclei including their invagination, volume increase, chromatin condensation and degradation as well as formation of micronuclei showed by AO/EB and 4,6-diamidino-2-phenylindol staining was accompanied by changes including increase in conductivity of cell electrolytes secreted to culture media, decrease in the number of the G1- and G2-phase cells and appearance of fraction of hypoploid cells as the effect of DNA degradation without ladder formation. Decrease in the number of mitochondria and in the activity of cellular dehydrogenases, production of reactive oxygen species (ROS), appearance of small and then large lytic vacuoles and increase in the amount of cytosolic calcium ions were also observed. The PCD was also manifested by increased width and weight of apical fragments of roots as well as decreased length of cortex cells which led to shortening of the whole roots. The kinetin-induced PCD process was almost completely inhibited by adenine, an inhibitor of phosphoribosyl transferase, and mannitol, an inhibitor of ROS production. These cell-death hallmarks and pathway of this process suggested that the induction of kinetin-specific vacuolar type of death, expressed itself with similar intensity on both morphological and metabolic levels, was a transient protecting whole roots and whole seedlings against elimination.
机译：用a啶橙和溴化乙锭（AO / EB）进行的双荧光染色表明，处理了蚕豆。幼苗在根皮层细胞中具有激动素诱导的程序性细胞死亡（PCD）。 AO / EB和4,6-diamidino-2-phenylindol染色显示由核素的形态变化反映的激动素诱导的细胞死亡，包括核的内陷，体积增加，染色质凝结和降解以及微核的形成。增加了分泌到培养基中的细胞电解质的电导率，减少了G1和G2期细胞的数量，以及由于DNA降解而没有梯形形成的次倍体细胞分数的出现。还观察到线粒体数量的减少和细胞脱氢酶活性的降低，活性氧种类（ROS）的产生，溶质液泡大小的出现，以及随后溶质液泡大小的增加以及胞质钙离子量的增加。 PCD还表现为根的根尖片段的宽度和重量增加以及皮质细胞长度减少，从而导致整个根的缩短。激动素诱导的PCD过程几乎被磷酸核糖基转移酶的抑制剂腺嘌呤和ROS生成的抑制剂甘露醇几乎完全抑制。这些细胞死亡的标志和这一过程的途径表明，激动素特异性液泡型死亡的诱导，在形态学和代谢水平上均以相似的强度表达，是瞬时保护整个根和整个幼苗免于消除。

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