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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >DEN+2-AAF-induced multistep hepatotumorigenesis in Wistar rats: Supportive evidence and insights
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DEN+2-AAF-induced multistep hepatotumorigenesis in Wistar rats: Supportive evidence and insights

机译：DEN + 2-AAF诱导的Wistar大鼠多步肝癌发生：支持性证据和见解

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摘要

Diethylnitrosamine (DEN), found in many commonly consumed foods, has been reported to induce cancers in animals and humans. Several models have been developed to study multistage carcinogenesis in rat liver; these include the Solt-Farber-resistant hepatocyte model. In the Solt-Farber model, the initiation consists of either a necrogenic dose of a hepatocarcinogen or a non-necrogenic dose in conjunction with partial hepatectomy (PH). We report a novel protocol for tumor induction in liver which eliminates the need for PH. Male Wistar rats were injected with single i. p. dose of DEN (200 mg/kg body weight), controls received saline only. After 1 week of recovery, the DEN-treated animals were administered with the repeated doses of 2-acetyamino fluorine (150 mg/kg body weight) orally in 1 % carboxymethyl cellulose that served as promoting agent. Thirty days after the DEN administration, hepatocellular damage was observed as evident by histopathological analysis. The marker enzyme analysis showed elevated levels of serum AST, ALT, and alkaline phosphatase and a decrease in the levels of liver superoxide dismutase and catalase. The oxidative stress in liver was confirmed by elevated levels of lipid peroxidation and a decrease in antioxidant parameters.
机译：据报道，在许多常见的食用食品中发现二乙基亚硝胺（DEN）会诱发动物和人类的癌症。已经开发了几种模型来研究大鼠肝脏的多阶段致癌作用。这些包括耐Solt-Farber的肝细胞模型。在Solt-Farber模型中，启动过程由致癌剂量的肝致癌物或非致死剂量的部分肝切除术（PH）组成。我们报告了一种在肝脏中诱导肿瘤的新方案，消除了对PH的需要。给雄性Wistar大鼠单次注射。 p。 DEN剂量（200 mg / kg体重），对照组仅接受生理盐水。恢复1周后，向DEN治疗的动物口服重复剂量的2-乙酰氨基氟（150mg / kg体重），在作为促进剂的1％羧甲基纤维素中。 DEN给药后三十天，通过组织病理学分析观察到肝细胞损伤。标记酶分析显示血清AST，ALT和碱性磷酸酶水平升高，肝脏超氧化物歧化酶和过氧化氢酶水平降低。脂质过氧化水平升高和抗氧化剂参数降低证实了肝脏的氧化应激。

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