首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Identification and characterization of superoxide dismutase in Phytophthora cinnamomi
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Identification and characterization of superoxide dismutase in Phytophthora cinnamomi

机译:肉桂疫霉中超氧化物歧化酶的鉴定与表征

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Superoxide dismutase (SOD) activities of the oomycete Phytophthora cinnamomi were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avocado SODs showed no evidence for the presence of either iron or copper/zinc SODs in P. cinnamomi. The level of activity of the MnSOD polypeptides decreased in the presence of avocado root or cell wall components. Growth of P. cinnamomi, measured as dry weight, increased when the mycelium was grown in the presence of superoxide anion (O_2 ~-), which was added exogenously. Our results suggest that the metabolism of O_2 ~- has an important role in the development of P. cinnamomi.
机译:检查了卵菌疫霉菌的超氧化物歧化酶(SOD)活性。在液体培养物中生长的菌丝体中发现了五种具有锰超氧化物歧化酶(MnSOD)活性的多肽,相对分子量约为25至100 kDa。与特征的鳄梨SOD的比较表明,没有证据表明肉桂粉中存在铁或铜/锌SOD。在鳄梨根或细胞壁成分的存在下,MnSOD多肽的活性水平降低。当菌丝体在外源添加的超氧阴离子(O_2〜-)存在下生长时,以干重衡量的肉桂假单胞菌的生长增加。我们的结果表明,O_2〜-的代谢在肉桂疫病的发展中具有重要作用。

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