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FRET-FLIM applications in plant systems

机译:FRET-FLIM在工厂系统中的应用

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摘要

A hallmark of cellular processes is the spatiotemporally regulated interplay of biochemical components. Assessing spatial information of molecular interactions within living cells is difficult using traditional biochemical methods. Developments in green fluorescent protein technology in combination with advances in fluorescence microscopy have revolutionised this field of research by providing the genetic tools to investigate the spatio-temporal dynamics of biomolecules in live cells. In particular, fluorescence lifetime imaging microscopy (FLIM) has become an inevitable technique for spatially resolving cellular processes and physical interactions of cellular components in real time based on the detection of F?rster resonance energy transfer (FRET). In this review, we provide a theoretical background of FLIM as well as FRETFLIM analysis. Furthermore, we show two cases in which advanced microscopy applications revealed many new insights of cellular processes in living plant cells as well as in whole plants.
机译:细胞过程的标志是生化成分的时空调控相互作用。使用传统的生化方法很难评估活细胞内分子相互作用的空间信息。绿色荧光蛋白技术的发展与荧光显微镜的发展相结合,通过提供遗传工具来研究活细胞中生物分子的时空动态,彻底改变了这一研究领域。尤其是,荧光寿命成像显微镜(FLIM)已成为基于Fster共振能量转移(FRET)的实时实时解析细胞过程和细胞成分物理相互作用的必然技术。在这篇综述中,我们提供了FLIM以及FRETFLIM分析的理论背景。此外,我们展示了两种情况,其中先进的显微镜应用揭示了活植物细胞以及整个植物中细胞过程的许多新见解。

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