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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Phenotypic and molecular characterisation of efficient nitrogen-fixing Azotobacter strains from rice fields for crop improvement
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Phenotypic and molecular characterisation of efficient nitrogen-fixing Azotobacter strains from rice fields for crop improvement

机译：稻田有效固氮固氮细菌菌株的表型和分子特性

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摘要

Biological nitrogen fixation (BNF) is highly effective in the field and potentially useful to reduce adverse effects chemical fertilisers. Here, Azotobacter species were selected via phenotypic, biochemical and molecular characterisations from different rice fields. Acetylene reduction assay of Azotobacter spp. showed that Azotobacter vinelandii (Az3) fixed higher amount of nitrogen (121.09 nmol C_2H_4mg~(-1) bacteria h~(-1)). Likewise, its plant growth functions, viz. siderophore, hydrogen cyanide, salicylic acid, IAA, GA3, zeatin, NH_3, phosphorus solubilisation, ACC deaminase and iron tolerance, were also higher. The profile of gDNA, plasmid DNA and cellular protein profile depicted inter-generic and inter-specific diversity among the isolates of A. vinelandii. The PCR-amplified genes nifH, nifD and nifK of 0.87, 1.4 and 1.5 kb, respectively, were ascertained by Southern blot hybridisation in isolates of A. vinelandii. The 16S rRNA sequence from A. vinelandii (Az3) was novel, and its accession number (JQ796077) was received from NCBI data base. Biofertiliser formulation of novel A. vinelandii isolates along with commercial one was evaluated in rice (Oriza sativa L. var. Khandagiri) fields. The present finding revealed that treatment T4 (Az3) (A. vinelandii) are highly efficient to improved growth and yield of rice crop.
机译：生物固氮（BNF）在该领域非常有效，并可能有助于减少化学肥料的不利影响。在这里，通过表型，生化和分子特征从不同稻田中选择了固氮菌物种。固氮菌属物种的乙炔还原测定。结果表明，葡萄固氮菌（Az3）固定了较高的氮含量（121.09 nmol C_2H_4mg〜（-1）细菌h〜（-1））。同样，其植物生长功能也就是。铁矿，氰化氢，水杨酸，IAA，GA3，玉米蛋白，NH_3，磷增溶，ACC脱氨酶和铁耐受性也更高。 gDNA，质粒DNA和细胞蛋白的概况描述了A.vinelandii分离株之间的种间和种间多样性。通过Southern印迹杂交在A.vinelandii的分离物中确定PCR扩增的基因nifH，nifD和nifK分别为0.87、1.4和1.5kb。来自A. vinelandii（Az3）的16S rRNA序列是新颖的，其登录号（JQ796077）从NCBI数据库获得。在稻田（Oriza sativa L. var。Khandagiri）中评估了新型A. vinelandii分离株的生物肥料配方和市售菌株。目前的发现表明，处理T4（Az3）（A。vinelandii）对提高水稻作物的生长和产量非常有效。

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