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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat
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Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat

机译：小麦成熟胚培养物中体外形态发生的形态组织学和基因型依赖性

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摘要

Cellular totipotency is one of the basic principles of plant biotechnology. Currently, the success of the procedure used to produce transgenic plants is directly proportional to the successful insertion of foreign DNA into the genome of suitable target tissue/cells that are able to regenerate plants. The mature embryo (ME) is increasingly recognized as a valuable explant for developing regenerable cell lines in wheat biotechnology. We have previously developed a regeneration procedure based on fragmented ME in vitro culture. Before we can use this regeneration system as a model for molecular studies of the morphogenic pathway induced in vitro and investigate the functional links between regenerative capacity and transformation receptiveness, some questions need to be answered. Plant regeneration from cultured tissues is genetically controlled. Factors such as age/degree of differentiation and physiological conditions affect the response of explants to culture conditions. Plant regeneration in culture can be achieved through embryogenesis or organogenesis. In this paper, the suitability of ME tissues for tissue culture and the chronological series of morphological data observed at the macroscopic level are documented. Genetic variability at each step of the regeneration process was evaluated through a varietal comparison of several elite wheat cultivars. A detailed histological analysis of the chronological sequence of morphological events during ontogeny was conducted. Compared with cultures of immature zygotic embryos, we found that the embryogenic pathway occurs slightly earlier and is of a different origin in our model. Cytological, physiological, and some biochemical aspects of somatic embryo formation in wheat ME culture are discussed.
机译：细胞全能是植物生物技术的基本原理之一。当前，用于生产转基因植物的方法的成功与成功地将外源DNA成功插入能够再生植物的合适靶组织/细胞的基因组成正比。成熟的胚胎（ME）日益被认为是开发小麦生物技术中可再生细胞系的有价值的外植体。我们先前已经开发了基于碎片化ME体外培养的再生程序。在我们可以将此再生系统用作体外诱导的形态发生途径的分子研究模型并研究再生能力和转化接受性之间的功能联系之前，需要回答一些问题。来自培养组织的植物再生是遗传控制的。年龄/分化程度和生理条件等因素会影响外植体对培养条件的反应。培养中的植物再生可通过胚胎发生或器官发生来实现。在本文中，记录了ME组织对组织培养的适用性以及在宏观水平上观察到的时间序列形态学数据。通过对几种优良小麦品种的品种比较，评估了再生过程每个步骤的遗传变异性。对个体发育过程中形态学事件的时间顺序进行了详细的组织学分析。与未成熟合子胚的培养相比，我们发现胚发生途径发生的时间稍早，并且在我们的模型中起源不同。讨论了小麦ME培养中体细胞胚形成的细胞学，生理学和某些生化方面。

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