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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The nitrate reductase inhibitor, tungsten, disrupts actin microfilaments in Zea mays L.
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The nitrate reductase inhibitor, tungsten, disrupts actin microfilaments in Zea mays L.

机译：硝酸还原酶抑制剂钨破坏了玉米中的肌动蛋白微丝。

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摘要

Tungsten is a widely used inhibitor of nitrate reductase, applied to diminish the nitric oxide levels in plants. It was recently shown that tungsten also has heavy metal attributes. Since information about the toxic effects of tungsten on actin is limited, and considering that actin microfilaments are involved in the entry of tungsten inside plant cells, the effects of tungsten on them were studied in Zea mays seedlings. Treatments with sodium tungstate for 3, 6, 12 or 24 h were performed on intact seedlings and seedlings with truncated roots. Afterwards, actin microfilaments in meristematic root and leaf tissues were stained with fluorescent phalloidin, and the specimens were examined by confocal laser scanning microscopy. While the actin microfilament network was well organized in untreated seedlings, in tungstate-treated ones it was disrupted in a time-dependent manner. In protodermal root cells, the effects of tungsten were stronger as cortical microfilaments were almost completely depolymerized and the intracellular ones appeared highly bundled. Fluorescence intensity measurements confirmed the above results. In the meristematic leaf tissue of intact seedlings, no depolymerization of actin microfilaments was noticed. However, when root tips were severed prior to tungstate application, both cortical and endoplasmic actin networks of leaf cells were disrupted and bundled after 24 h of treatment. The differential response of root and leaf tissues to tungsten toxicity may be due to differential penetration and absorption, while the effects on actin microfilaments could not be attributed to the nitric oxide depletion by tungsten.
机译：钨是广泛使用的硝酸盐还原酶抑制剂，可用于降低植物中的一氧化氮水平。最近显示，钨还具有重金属属性。由于有关钨对肌动蛋白毒性作用的信息有限，并且考虑到肌动蛋白微丝参与植物细胞内部钨的进入，因此在玉米幼苗中研究了钨对肌动蛋白的影响。在完整的幼苗和具有截短的根的幼苗上进行钨酸钠处理3、6、12或24小时。然后，用荧光鬼笔环肽对分生组织的根和叶组织中的肌动蛋白微丝染色，并用共聚焦激光扫描显微镜检查标本。肌动蛋白微丝网络在未经处理的幼苗中组织良好，而在经过钨酸盐处理的幼苗中，肌动蛋白微丝网络以时间依赖性方式被破坏。在原皮根细胞中，钨的作用更强，因为皮质微丝几乎完全解聚，而胞内微丝似乎高度束缚。荧光强度测量证实了以上结果。在完整幼苗的分生组织中，未观察到肌动蛋白微丝的解聚。但是，当在施用钨酸盐之前切断根尖时，处理后24小时，叶细胞的皮质和内质肌动蛋白网络都被破坏并捆绑在一起。根和叶组织对钨毒性的不同反应可能是由于渗透和吸收的差异，而对肌动蛋白微丝的影响不能归因于钨对一氧化氮的消耗。

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