Serial block face scanning electron microscopy-the future of cell ultrastructure imaging

Louise Hughes; Chris Hawes; Sandy Monteith; Sue Vaughan

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Serial block face scanning electron microscopy-the future of cell ultrastructure imaging

机译：串行块面扫描电子显微镜-细胞超微结构成像的未来

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One of the major drawbacks in transmission electron microscopy has been the production of three-dimensional views of cells and tissues. Currently, there is no one suitable 3D microscopy technique that answers all questions and serial block face scanning electron microscopy (SEM) fills the gap between 3D imaging using high-end fluorescence microscopy and the high resolution offered by electron tomography. In this review, we discuss the potential of the serial block face SEM technique for studying the three-dimensional organisation of animal, plant and microbial cells.

机译：透射电子显微镜的主要缺点之一是产生细胞和组织的三维视图。当前，没有一种合适的3D显微镜技术能够回答所有问题，而串行块面扫描电子显微镜（SEM）填补了高端荧光显微镜在3D成像与电子断层扫描提供的高分辨率之间的空白。在这篇综述中，我们讨论了连续块面扫描电子显微镜技术在研究动物，植物和微生物细胞的三维组织方面的潜力。

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《Protoplasma: An International Journal of Cell Biology》 |2014年第2期|共7页
作者
Louise Hughes; Chris Hawes; Sandy Monteith; Sue Vaughan;
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正文语种 eng
中图分类细胞形态学;
关键词
Serial block face scanning electronmicroscopy; SBEM; Selective staining; 3D reconstruction;

机译：连续块面扫描电子显微镜;SBEM;选择性染色;3D重建;

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1. Serial block face scanning electron microscopy-the future of cell ultrastructure imaging [J] . Louise Hughes, Chris Hawes, Sandy Monteith, Protoplasma: An International Journal of Cell Biology . 2014,第2期

机译：串行块面扫描电子显微镜-细胞超微结构成像的未来
2. Segmentation and Modelling of the Nuclear Envelope of HeLa Cells Imaged with Serial Block Face Scanning Electron Microscopy ? [J] . Cefa Karaba?, Martin L. Jones, Christopher J. Peddie, Journal of Imaging . 2019,第9期

机译：用串联块面扫描电子显微镜成像的HeLa细胞核包膜的分割和建模？
3. Serial block face scanning electron microscopy for the study of cardiac muscle ultrastructure at nanoscale resolutions [J] . Pinali Christian, Kitmitto Ashraf Journal of Molecular and Cellular Cardiology . 2014,第Null期

机译：串联块面扫描电子显微镜用于纳米级分辨率的心肌超微结构研究
4. Placental Alkaline Phosphatase Labeling for Tracing Transgenic Mouse Retinal Bipolar Cells in 3D Serial Blockface Scanning Electron Microscopy Datasets [C] . Sharon Frase, Amir Avishai, Nanthawan Avishai, Microscopy Society of America Annual Meeting;Microanalysis Society Annual meeting;International Metallographic Society Annual meeting . 2012

机译：胎盘碱性磷酸酶标记，用于跟踪3D串行面部扫描电子显微镜数据集中的转基因小鼠视网膜双极细胞。
5. Automated sub-micron resolution serial block face imaging of cancellous bone using epifluorescence microscopy. [D] . Slyfield, Craig Richard, Jr. 2008

机译：使用落射荧光显微镜对松质骨进行自动亚微米分辨率串行块面部成像。
6. Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning electron microscopic images [O] . James C. Bouwer, Thomas J. Deerinck, Eric Bushong, -1

机译：载物台偏置电位对探测光束的减速提高了串行块面扫描电子显微镜图像的分辨率
7. Serial block face scanning electron microscopy for the study of cardiac muscle ultrastructure at nanoscale resolutions [O] . Pinali Christian, Kitmitto Ashraf 2014

机译：串联块面扫描电子显微镜用于纳米级分辨率的心肌超微结构研究

Serial block face scanning electron microscopy-the future of cell ultrastructure imaging

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