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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Production of anthraquinones, phenolic compounds and biological activities from hairy root cultures of Polygonum multiflorum Thunb.
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Production of anthraquinones, phenolic compounds and biological activities from hairy root cultures of Polygonum multiflorum Thunb.

机译：何首乌毛状根培养物中蒽醌，酚类化合物的生产和生物活性。

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Polygonum multiflorum Thunb. is a highly important medicinal plant producing anthraquinones (emodin and physcion) and phenolic compounds which has pharmaceutical use. In vitro seedling explants such as roots, internodals, nodals and leaves were inoculated with A. rhizogenes strain KCTC 2703. Transformed roots were induced from internodals and leaf explants. Six transgenic clones of hairy roots were established and confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) using rolC specific primers. Hairy roots cultured using MS liquid medium supplemented with 30 g/l sucrose showed highest accumulation of biomass (99.05 g/l FW [fresh weight] and 10.95 g/l DW [dry weight]) and highest production of anthraquinones content (emodin 211.32 μg/g DW and physcion 353.23 μg/g DW) were observed at 20 days. Nearly 9.5-fold increment of biomass was evident in suspension cultures at 20 days of culture and hairy root biomass produced in suspension cultures possessed 3.7- and 3.5-fold higher content of emodin and physcion, respectively, when compared with the untransformed control roots. MS basal liquid medium was superior for the growth of hairy roots and production of anthraquinones compared with other culturemedia evaluated (SH, B5 and N6), with MS-basal liquid medium supplemented with 30 g/l sucrose was optimal for secondary metabolite production. A total of 23 polyphenolic compounds were identified and quantified from P. multiflorum untransformed and hairy roots, which includes hydroxybenzoic acids, hydroxycinnamic acids, flavonols and other groups of phenolic compounds. The ultra-performance liquid chromatography (UPLC) analysis of the phenolic compounds profile revealed that pyrogallol, hesperidin, naringenin and formononetin were higher in hairy roots compared to untransformed roots. The total phenolics, flavonoids content, antioxidant and antimicrobial activity was high in hairy roots compared to untransformed roots. This is the first report for the production of anthraquinones (emodin and physcion), phenolic compounds and biological activities from hairy root cultures of P. multiflorum.
机译：multi何首乌。是生产蒽醌（大黄素和physcion）和酚类化合物的高度重要的药用植物，具有药用。用发根农杆菌KCTC 2703接种体外幼苗外植体，例如根，节间，节和叶。从间节和叶外植体中诱导转化根。建立了六个毛状根的转基因克隆，并使用rolC特异性引物通过聚合酶链反应（PCR）和逆转录PCR（RT-PCR）进行了确认。使用补充了30 g / l蔗糖的MS液体培养基培养的毛状根表现出最高的生物量积累（99.05 g / l FW [鲜重]和10.95 g / l DW [干重]），并且蒽醌含量最高（大黄素211.32μg）在20天时观察到/ g DW和physcion 353.23μg/ g DW）。与未转化的对照根相比，悬浮培养在培养20天时生物量增加了近9.5倍，悬浮培养中产生的毛根生物量大黄素和physcion的含量分别高3.7和3.5倍。与其他评估的培养基（SH，B5和N6）相比，MS基础液体培养基在毛状根的生长和蒽醌的产生方面更胜一筹，而MS基础液体培养基中补充有30 g / l蔗糖是次生代谢产物的最佳选择。从何首乌未转化和毛状根中鉴定和定量了总共23种多酚化合物，包括羟基苯甲酸，羟基肉桂酸，黄酮醇和其他酚类化合物。超高效液相色谱（UPLC）对酚类化合物谱的分析表明，毛状根中的邻苯三酚，橙皮苷，柚皮苷和甲萘醌的转化率高于未转化根。与未转化的根相比，毛状根的总酚，类黄酮含量，抗氧化剂和抗菌活性较高。这是关于何首乌毛状根培养物中蒽醌（大黄素和physcion），酚类化合物的生产和生物活性的首次报道。

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