...
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Quantifying intracellular dynamics using fluorescence fluctuation spectroscopy
【24h】

Quantifying intracellular dynamics using fluorescence fluctuation spectroscopy

机译:使用荧光波动光谱法定量细胞内动力学

获取原文
获取原文并翻译 | 示例
           

摘要

Originally developed for the field of physical chemistry, fluorescence fluctuation spectroscopy (FFS) has evolved to a family of methods to quantify concentrations, diffusion rates and interactions of fluorescently labelled molecules. The possibility to measure at the nanomolar concentration level and to combine these techniqueswithmicroscopy allow to study biological processes with high sensitivity in the living cell. In this review, the basic principles, challenges and recent developments of the most common FFS methods are being discussed and illustrated by intracellular applications.
机译:荧光波动光谱法(FFS)最初是为物理化学领域开发的,现已发展为量化荧光标记分子的浓度,扩散速率和相互作用的一系列方法。在纳摩尔浓度水平上进行测量并将这些技术与显微镜相结合的可能性允许研究在活细胞中具有高灵敏度的生物学过程。在这篇综述中,最常见的FFS方法的基本原理,挑战和最新发展正在通过细胞内应用进行讨论和说明。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号