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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Fluorescence lifetime imaging microscopy in the medical sciences
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Fluorescence lifetime imaging microscopy in the medical sciences

机译：医学科学中的荧光寿命成像显微镜

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The steady improvement in the imaging of cellular processes in living tissue over the last 10-15 years through the use of various fluorophores including organic dyes, fluorescent proteins and quantum dots, has made observing biological events common practice. Advances in imaging and recording technology have made it possible to exploit a fluorophore's fluorescence lifetime. The fluorescence lifetime is an intrinsic parameter that is unique for each fluorophore, and that is highly sensitive to its immediate environment and/or the photophysical coupling to other fluorophores by the phenomenon F?rster resonance energy transfer (FRET). The fluorescence lifetime has become an important tool in the construction of optical bioassays for various cellular activities and reactions. The measurement of the fluorescence lifetime is possible in two formats; time domain or frequency domain, eachwith their own advantages. Fluorescence lifetime imaging applications have now progressed to a state where, besides their utility in cell biological research, they can be employed as clinical diagnostic tools. This review highlights the multitude of fluorophores, techniques and clinical applications that make use of fluorescence lifetime imaging microscopy (FLIM).
机译：在过去的10至15年中，通过使用包括有机染料，荧光蛋白和量子点在内的各种荧光团，对活组织中细胞过程成像的稳步提高，已使观察生物事件成为普遍的做法。成像和记录技术的进步使得开发荧光团的荧光寿命成为可能。荧光寿命是每个荧光团唯一的固有参数，并且通过现象共振共振能量转移（FRET）对它的直接环境和/或与其他荧光团的光物理耦合高度敏感。荧光寿命已成为构建用于各种细胞活动和反应的光学生物测定的重要工具。荧光寿命的测量有两种格式：时域或频域，各有千秋。荧光寿命成像应用现在已经发展到一种状态，除了在细胞生物学研究中的用途外，它们还可以用作临床诊断工具。这篇综述重点介绍了利用荧光寿命成像显微镜（FLIM）的众多荧光团，技术和临床应用。

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