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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Isolation and characterisation of the trichocysts of the dinophyte Prorocentrum micans
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Isolation and characterisation of the trichocysts of the dinophyte Prorocentrum micans

机译：附生植物原生圆球菌的毛囊的分离与鉴定

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摘要

Trichocyst-enriched fractions were isolated from the marine dinophyte Prorocentrum micans. Transmission electron microscopy revealed that most of the trichocysts were discharged and had elongated to long filaments. Some trichocysts were still condensed. Fragments of discharged trichocysts measured up to 20 mu m in length and 260 nm in width, those still condensed measured up to 1 mu m in width and 16 mu m in length. A distinct banding pattern with a transversal periodicity of approximately 16-18 nm and a periodic longitudinal striation of 3-4 nm could be measured along the trichocyst filaments. At higher magnifications, a fragile, alveolated, net-like organisation became obvious which resembled the one shown for the trichocysts of ciliates. When trichocyst-enriched fractions were treated with sodium dodecyl sulfate and centrifuged subsequently, no trichocysts were registered any longer in the sodium dodecyl sulfate-insoluble fraction by electron microscopy. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of trichocyst-enriched fractions and of the SDS-soluble fractions revealed a protein banding pattern which was dominated by polypeptides of 50-30, 12.5, and approximately 8.5 kDa. The polypeptide banding pattern deviated significantly from those registered for ejectisomes of cryptophytes and of the prasinophyte Pyramimonas grossii, for the Reb polypeptides which constitute the R-bodies of Caedibacter taeniospiralis, and also from the banding pattern of trichocysts of Paramecium. An antiserum directed against trichocysts of Paramecium did not cross-react with the polypeptides present in the trichocyst-enriched fraction of Prorocentrum micans.
机译：从海洋藻类植物原位轮虫中分离出富含Trichocyst的级分。透射电子显微镜显示，大多数毛囊均被释放，并已伸长至长丝状。一些毛囊仍在凝结。排出的毛状囊的碎片的长度最长为20微米，宽度为260 nm，仍然凝结的碎片的宽度最大为1微米，长度为16微米。沿着毛囊丝可以测量到一个明显的带状图样，其横向周期性约为16-18 nm，周期性纵向条纹为3-4 nm。在更高的放大倍数下，一个脆弱的，蜂窝状的，网状组织变得明显，类似于纤毛虫的毛囊。当用十二烷基硫酸钠处理富含毛囊的级分并随后离心时，通过电子显微镜检查，在十二烷基硫酸钠不溶性级分中不再记录任何毛囊。富含毛囊的级分和SDS可溶级分的十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示出蛋白条带模式，该条带模式由50-30、12.5和大约8.5 kDa的多肽主导。多肽条带模式显着不同于隐藻类和拟南芥的Pyramimonas Grossii的射出同质体，构成taeniospiralis的R体的Reb多肽，以及偏离草履虫的单毛囊的带状模式。针对草履虫的毛囊囊的抗血清未与富含原肠毛虫的毛囊中的部分中存在的多肽发生交叉反应。

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