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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Hairy root biotechnology-indicative timeline to understand missing links and future outlook
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Hairy root biotechnology-indicative timeline to understand missing links and future outlook

机译:有毛的根生物技术指示性时间表,以了解缺失的环节和未来展望

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Agrobacterium rhizogenes-mediated hairy roots (HR) were developed in the laboratory to mimic the natural phenomenon of bacterial gene transfer and occurrence of disease syndrome. The timeline analysis revealed that during 90 s, the research expanded to the hairy root-based secondary metabolite production and different yield enhancement strategies like media optimization, up-scaling, metabolic engineering etc. An outlook indicates that much emphasis has been given to the strategies that are helpful in making this technology more practical in terms of high productivity at low cost. However, a sequential analysis of literature shows that this technique is upgraded to a biotechnology platform where different intra- and interdisciplinary work areas were established, progressed, and diverged to provide scientific benefits of various hairy root-based applications like phytoremediation, molecular farming, biotransformation, etc. In the present scenario, this biotechnology research platform includes (a) elemental research like hairy root-mediated secondary metabolite production coupled with productivity enhancement strategies and (b) HR-based functional research. The latter comprised of hairy root-based applied aspects such as generation of agro-economical traits in plants, production of high value as well as less hazardous molecules through biotransformation/farming and remediation, respectively. This review presents an indicative timeline portrayal of hairy root research reflected by a chronology of research outputs. The timeline also reveals a progressive trend in the state-of-art global advances in hairy root biotechnology. Furthermore, the review also discusses ideas to explore missing links and to deal with the challenges in future progression and prospects of research in all related fields of this important area of plant biotechnology.
机译:在实验室中开发了发根农杆菌介导的毛状根(HR),以模仿细菌基因转移和疾病综合征发生的自然现象。时间线分析显示,在90年代,研究扩展到了基于毛根的次生代谢产物生产以及不同的增产策略,如培养基优化,放大,代谢工程等。前景表明,该策略已得到了更多的重视这有助于使该技术在低成本,高生产率方面更加实用。但是,对文献的顺序分析表明,该技术已升级到一个生物技术平台,在该平台上建立,发展和分散了不同的学科和跨学科工作领域,从而为基于植物修复,分子耕作,生物转化等各种基于毛根的应用提供了科学益处。在当前情况下,此生物技术研究平台包括(a)基础研究,例如毛状根介导的次生代谢产物生产以及生产率提高策略,以及(b)基于HR的功能研究。后者包括基于毛发根的应用方面,例如通过生物转化/农业和修复分别在植物中产生农业经济性状,产生高价值以及危害较小的分子。这篇评论提出了由研究成果的时间顺序反映的有毛根研究的指示性时间表。该时间表还揭示了有毛根生物技术的最新全球进步趋势。此外,本综述还讨论了一些想法,以探索缺失的环节,并应对这一重要的植物生物技术领域的所有相关领域中的未来发展挑战和研究前景。

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