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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Improved callus induction, shoot regeneration, and salt stress tolerance in Arabidopsis overexpressing superoxide dismutase from Potentilla atrosanguinea
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Improved callus induction, shoot regeneration, and salt stress tolerance in Arabidopsis overexpressing superoxide dismutase from Potentilla atrosanguinea

机译：改良过表达委陵菜委陵菜超氧化物歧化酶的拟南芥的愈伤组织诱导，芽再生和盐胁迫耐受性

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Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals (O-2 (center dot) (-)) to molecular oxygen (O-2) and hydrogen peroxide (H2O2). Previously, we have identified and characterized a thermo-tolerant copper-zinc superoxide dismutase from Potentilla atrosanguinea (PaSOD), which retains its activity in the presence of NaCl. In the present study, we show that cotyledonary explants of PaSOD overexpressing transgenic Arabidopsis thaliana exhibit early callus induction and high shoot regenerative capacity than wild-type (WT) explants. Growth kinetic studies showed that transgenic lines have 2.6-3.3-folds higher growth rate of calli compared to WT. Regeneration frequency of calli developed from transgenic cotyledons was found to be 1.5-2.5-folds higher than that of WT explants on Murashige and Skoog medium supplemented with different concentrations of naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) within 2 weeks. A positive regulatory effect of PaSOD and H2O2 was observed on different stages of callusing and regeneration. However, this effect was more pronounced at the early stages of the regeneration processes in transgenic lines as compared to WT. These results clearly indicate that plant regeneration is regulated by endogenous H2O2 and by factors, which enhance its accumulation. Transgenics also exhibited salt stress tolerance with higher SOD activity, chlorophyll content, total soluble sugars, and proline content, while lower ion leakage and less reduction in relative water content, as compared to WT. Thus, it appears that the activation of PaSOD at regeneration stage accompanied by increased H2O2 production can be one of the mechanisms controlling in vitro morphogenesis.
机译：超氧化物歧化酶（SOD）催化超氧化物自由基（O-2（中心点）（-））转变为分子氧（O-2）和过氧化氢（H2O2）。以前，我们已经鉴定并鉴定了来自委陵菜委陵菜（PaSOD）的耐热铜锌超氧化物歧化酶，该酶在NaCl存在下仍能保持其活性。在本研究中，我们显示过表达PaSOD的子叶外植体比野生型（WT）外植体表现出早期的愈伤组织诱导和较高的芽再生能力。生长动力学研究表明，与WT相比，转基因品系的愈伤组织生长速率高2.6-3.3倍。发现转基因子叶发育的愈伤组织的再生频率比在补充有不同浓度的萘乙酸（NAA）和6-苄基氨基嘌呤（BAP）的Murashige和Skoog培养基上的WT外植体高1.5-2.5倍。在愈伤组织和再生的不同阶段，观察到PaSOD和H2O2的正调节作用。然而，与WT相比，该效果在转基因品系的再生过程的早期更加明显。这些结果清楚地表明植物再生受内源性过氧化氢和增强其积累的因素的调节。与WT相比，转基因产品还表现出耐盐胁迫性，具有较高的SOD活性，叶绿素含量，总可溶性糖和脯氨酸含量，而离子泄漏较少，相对含水量减少较少。因此，似乎PaSOD在再生阶段的活化伴随着H2O2产量的增加可能是控制体外形态发生的机制之一。

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