...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation
【24h】

Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation

机译：不同罗汉果愈伤组织培养物中抗癌三萜烯（山梨酸）的产生及其诱因

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Betulinic acid (BA), a pentacyclic triterpenoid, is gaining unmatched attention owing to its unique anti-cancer activity with selective melanoma growth inhibition without damaging normal cells. It is also well-known for its multifaceted pharmacokinetics, entailing antibacterial, antimalarial, anti-HIV and antioxidant merits. Considering the escalating demand with diminishing bioresource of this molecule, the present study was undertaken that revealed the untapped potentials of Ocimum calli, contrasting to that in the in vitro derived leaves, as effective production alternative of BA in three out of four tested species (i.e. Ocimum basilicum, Ocimum kilimandscharicum, Ocimum sanctum excluding Ocimum grattisimum). Callus inductions were obtained in all the four species with different 2,4-dichlorophenoxyacetic acid (2,4-D)/alpha-naphthaleneacetic acid (NAA) concentrations with kinetin. Notably, 2,4-D favoured maximum callus growth in all whereas NAA proved beneficial for the highest metabolite yield in the calli of each BA-producing species. The O. basilicum calli demonstrated the maximum growth (growth index (GI) 678.7 +/- 24.47) and BA yield (2.59 +/- 0.55 % dry weight [DW]), whereas those in O. kilimandscharicum (GI 533.33 +/- 15.87; BA 1.87 +/- 0.6 % DW) and O. sanctum (GI 448 +/- 16.07; BA 0.39 +/- 0.12 % DW) followed a descending order. The O. gratissimum calli revealed minimum growth (GI 159 +/- 13.25) with no BA accumulation. Elicitation with methyl jasmonate at 200-mu M concentration after 48-h exposure doubled the BA yield (5.10 +/- 0.18 % DW) in NAA-grown O. basilicum calli compared to that in the untreated counterpart (2.61 +/- 0.19 % DW), which further enthused its future application.
机译：五环三萜类化合物桦木酸（BA）由于具有独特的抗癌活性和选择性的黑色素瘤生长抑制作用而不会损害正常细胞，因此受到了无与伦比的关注。它也因其多方面的药代动力学而闻名，具有抗菌，抗疟疾，抗艾滋病毒和抗氧化的优点。考虑到该分子的生物资源减少，需求不断增加，因此进行了本研究，揭示了Ocimum愈伤组织的未开发潜力，与体外衍生叶片的潜力相反，它是四种测试物种中三种中BA的有效生产替代品（即罗勒罗勒（Ocimum basilicum），罗勒（Kilimandscharicum）罗勒（Ocimum kilimandscharicum），圣罗勒（Ocimum Sanctum）（不包括罗汉果（Ocimum grattisimum）。在所有四个物种中，以不同的2，4-二氯苯氧基乙酸（2,4-D）/α-萘乙酸（NAA）浓度与动蛋白进行愈伤组织诱导。值得注意的是，2，4-D有利于所有愈伤组织的最大生长，而NAA被证明有利于每个BA生产物种的愈伤组织中最高的代谢产物产量。罗勒油菜愈伤组织表现出最大的生长（生长指数（GI）678.7 +/- 24.47）和BA产量（2.59 +/- 0.55％干重[DW]），而在基里奇菜（O. kilimandscharicum）（GI 533.33 +/-） 15.87; BA 1.87 +/- 0.6％DW）和密室（GI 448 +/- 16.07; BA 0.39 +/- 0.12％DW）降序排列。东方乳杆菌显示最小生长（GI 159 +/- 13.25），没有BA积累。暴露48小时后，用200 um M浓度的茉莉酸甲酯进行激化，与未处理的茉莉酸橙愈伤组织相比，未处理的茉莉酸愈伤组织的BA产量翻倍（5.10 +/- 0.18％DW）（2.61 +/- 0.19％） DW），这进一步促进了其未来的应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号