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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Regulation of some salt defense-related genes in relation to physiological and biochemical changes in three sugarcane genotypes subjected to salt stress
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Regulation of some salt defense-related genes in relation to physiological and biochemical changes in three sugarcane genotypes subjected to salt stress

机译：三种盐胁迫下甘蔗基因型中一些与盐分防御相关的基因与生理生化变化的关系

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Sugarcane (Saccharum officinale L.; Poaceae) is a sugar-producing plant widely grown in tropic. Being a glycophytic species, it is very sensitive to salt stress, and salinity severely reduces growth rate and cane yield. The studies investigating the regulation of salt defense metabolite-related genes in relation to final biochemical products in both susceptible and tolerant genotypes of sugarcane are largely lacking. We therefore investigated the expression levels of sugarcane shaggy-like kinase (SuSK), sucrose transporter (SUT), proline biosynthesis (pyrolline-5-carboxylate synthetase; P5CS), ion homeostasis (NHX1), and catalase (CAT2) mRNAs, and contents of Na+, soluble sugar, and free proline in three sugarcane genotypes (A19 mutant, K88-92, and K92-80) when subjected to salt stress (200 mM NaCl). The relative expression levels of salt defense-related genes in salt-stressed plantlets of sugarcane cv. K88-92 were upregulated in relation to salt exposure times when compared with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as housekeeping gene. In addition, final biochemical products, i.e., low Na+, sucrose enrichment, and free proline accumulation, were evidently demonstrated in salt-stressed plantlets. Chlorophyll b, total chlorophyll, total carotenoid concentrations, and maximum quantum yield of PSII (F (v)/F (m)) in positive check (K88-92) were maintained under salt stress, leading to high net photosynthetic rate (P (n)) and growth retention (root length, fresh weight, and leaf area). In contrast, photosynthetic abilities in negative check, K92-80, and A19 mutant lines grown under salt stress declined significantly in comparison to control, leading to a reduction in P (n) and an inhibition of overall growth characters. The study concludes that the genetic background of sugarcane cv. K88-92 may further be exploited to play a key role as parental clone for sugarcane breeding program for salt-tolerant purposes.
机译：甘蔗（Saccharum officinale L .;禾本科）是一种在热带地区广泛种植的产糖植物。作为糖类物种，它对盐胁迫非常敏感，盐度严重降低了生长速率和甘蔗产量。缺乏关于甘蔗易感和耐性基因型中与最终生化产物有关的盐防御代谢物相关基因的调控的研究。因此，我们研究了甘蔗蓬松状激酶（SuSK），蔗糖转运蛋白（SUT），脯氨酸生物合成（吡咯烷5-羧酸合成酶; P5CS），离子稳态（NHX1）和过氧化氢酶（CAT2）mRNA的表达水平和含量盐胁迫（200 mM NaCl）时三种甘蔗基因型（A19突变体，K88-92和K92-80）中的Na +，可溶性糖和游离脯氨酸的含量。盐胁迫甘蔗小植株中盐分防御相关基因的相对表达水平。与作为保管基因的甘油醛-3-磷酸脱氢酶（GAPDH）相比，K88-92在盐接触时间方面上调。另外，在盐胁迫的小植株中显然证明了最终的生化产物，即低Na +，蔗糖富集和游离脯氨酸的积累。在盐胁迫下维持阳性对照（K88-92）中的叶绿素b，总叶绿素，总类胡萝卜素浓度和PSII的最大量子产率（F（v）/ F（m）），从而导致较高的净净光合速率（P（ n））和生长保持力（根长，鲜重和叶面积）。相反，与对照相比，在盐胁迫下生长的阴性对照，K92-80和A19突变株的光合能力显着下降，导致P（n）降低并抑制了总体生长特性。研究得出的结论是甘蔗简历的遗传背景。 K88-92可以进一步被用作甘蔗育种计划的父母克隆，以达到耐盐的目的。

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