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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition
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Confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition

机译：共聚焦激光扫描显微镜阐明叶片角质层的微观形态及其化学成分分析

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摘要

Electron microscopy techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) have been invaluable tools for the study of the micromorphology of plant cuticles. However, for electron microscopy, the preparation techniques required may invariably introduce artefacts in cuticle preservation. Further, there are a limited number of methods available for quantifying the image data obtained through electron microscopy. Therefore, in this study, optical microscopy techniques were coupled with staining procedures and, along with SEM were used to qualitatively and quantitatively assess the ultrastructure of plant leaf cuticles. Leaf cryosections of Triticum aestivum (wheat), Zea mays (maize), and Lupinus angustifolius (lupin) were stained with either fat-soluble azo stain Sudan IV or fluorescent, diarylmethane Auramine O and were observed under confocal laser scanning microscope (CLSM). For all the plant species tested, the cuticle on the leaf surfaces could be clearly resolved in many cases into cuticular proper (CP), external cuticular layer (ECL), and internal cuticular layer (ICL). Novel image data analysis procedures for quantifying the epicuticular wax micromorphology were developed, and epicuticular waxes of L. angustifolius were described here for the first time. Together, application of a multifaceted approach involving the use of a range of techniques to study the plant cuticle has led to a better understanding of cuticular structure and provides new insights into leaf surface architecture.
机译：电子显微镜技术，例如透射电子显微镜（TEM）和扫描电子显微镜（SEM），已成为研究植物角质层微观形态的宝贵工具。但是，对于电子显微镜，所需的制备技术可能会在表皮保存过程中始终引入假象。此外，有限数量的方法可用于量化通过电子显微镜获得的图像数据。因此，在这项研究中，光学显微镜技术与染色程序相结合，并与SEM一起用于定性和定量评估植物叶片表皮的超微结构。小麦（小麦），玉米（玉米）和羽扇豆（羽扇豆）的叶片冷冻切片用脂溶性偶氮染料Sudan IV或荧光二芳基甲烷金胺O染色，并在共聚焦激光扫描显微镜（CLSM）下观察。对于所有测试的植物物种，在许多情况下，叶片表面的表皮可以清楚地分解为表皮固有层（CP），表皮外层（ECL）和表皮内层（ICL）。开发了用于量化表皮蜡微观形态的新型图像数据分析程序，并在此首次描述了古铜色的表皮蜡。总之，采用涉及多种技术来研究植物角质层的多方面方法的应用，导致人们对角质层结构有了更好的了解，并为叶片表面结构提供了新的见识。

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