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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The sexual phase of the diatom Pseudo-nitzschia multistriata: cytological and time-lapse cinematography characterization
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The sexual phase of the diatom Pseudo-nitzschia multistriata: cytological and time-lapse cinematography characterization

机译：硅藻假线虫的性期：细胞学和延时摄影的特征

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摘要

Pseudo-nitzschia is a thoroughly studied pennate diatom genus for ecological and biological reasons. Many species in this genus, including Pseudo-nitzschia multistriata, can produce domoic acid, a toxin responsible for amnesic shellfish poisoning. Physiological, phylogenetic and biological features of P. multistriata were studied extensively in the past. Life cycle stages, including the sexual phase, fundamental in diatoms to restore the maximum cell size and avoid miniaturization to death, have been well described for this species. P. multistriata is heterothallic; sexual reproduction is induced when strains of opposite mating type are mixed, and proceeds with cells producing two functionally anisogamous gametes each; however, detailed cytological information for this process is missing. By means of confocal laser scanning microscopy and nuclear staining, we followed the nuclear fate during meiosis, and using time-lapse cinematography, we timed every step of the sexual reproduction process from mate pairing to initial cell hatching. The present paper depicts cytological aspects during gametogenesis in P. multistriata, shedding light on the chloroplast behaviour during sexual reproduction, finely describing the timing of the sexual phases and providing reference data for further studies on the molecular control of this fundamental process.
机译：由于生态和生物学原因，拟南芥属是一种经过充分研究的戊二烯硅藻属。该属中的许多物种，包括假拟南芥，都可以产生海藻酸，这是一种导致遗忘性贝类中毒的毒素。过去广泛研究了P. multistriata的生理，系统发生和生物学特征。该物种已充分描述了硅藻生命周期阶段，包括性阶段，这是硅藻恢复最大细胞大小并避免小型化死亡所必需的。 P. multistriata是杂种的;当混合相反交配类型的菌株时，会诱导有性生殖，并继续进行，每个细胞会产生两个功能上异配的配子;但是，缺少此过程的详细细胞学信息。通过共聚焦激光扫描显微镜和核染色，我们观察了减数分裂过程中的核命运，并使用延时摄影技术对从伴侣配对到初始细胞孵化的性繁殖过程的每个步骤进行了计时。本文描述了多纹体育的配子发生过程中的细胞学方面，揭示了有性生殖过程中叶绿体的行为，精细描述了性相的时间，并为进一步研究该基本过程的分子控制提供了参考数据。

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