...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm
【24h】

Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm

机译：大麦胚乳中瞬时表达大麦ESCRT-III组分HvVPS60的细胞层特异性分布

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The significance of the endosomal sorting complexes required for transport (ESCRT)-III in cereal endosperm has been shown by the identification of the recessive mutant supernumerary aleurone layer1 (SAL1) in maize. ESCRT-III is indispensable in the final membrane fission step during biogenesis of multivesicular bodies (MVBs), responsible for protein sorting to vacuoles and to the cell surface. Here, we annotated barley ESCRT-III members in the (model) crop Hordeum vulgare and show that all identified members are expressed in developing barley endosperm. We used fluorescently tagged core ESCRT-III members HvSNF7a/CHMP4 and HvVPS24/CHMP3 and the associated ESCRT-III component HvVPS60a/CHMP5 for transient localization studies in barley endosperm. In vivo confocal microscopic analyses show that the localization of recombinantly expressed HvSNF7a, HvVPS24 and HvVPS60a differs within barley endosperm. Whereas HvSNF7a induces large agglomerations, HvVPS24 shows mainly cytosolic localization in aleurone and subaleurone. In contrast, HvVPS60a localizes strongly at the plasma membrane in aleurone. In subaleurone, HvVPS60a was found to a lesser extent at the plasma membrane and at vacuolar membranes. These results indicate that the steady-state association of ESCRT-III may be influenced by cell layer-specific protein deposition or trafficking and remodelling of the endomembrane system in endosperm. We show that sorting of an artificially mono-ubiquitinated Arabidopsis plasma membrane protein is inhibited by HvVPS60a in aleurone. The involvement of HvVPS60a in different cell layer-specific trafficking pathways, reflected by localization of HvVPS60a at the plasma membrane in aleurone and at the PSV membrane in subaleurone, is discussed.
机译：鉴定玉米胚乳中运输所需的内体分选复合物（ESCRT）-III的重要性已通过玉米隐性突变体多余糊粉层1（SAL1）的鉴定得到证明。 ESCRT-III是多囊体（MVB）生物合成过程中最后的膜裂变步骤中必不可少的部分，它负责将蛋白质分类为液泡和细胞表面。在这里，我们对（模型）农作物大麦中的大麦ESCRT-III成员进行了注释，并表明所有鉴定出的成员均在发育中的大麦胚乳中表达。我们将荧光标记的核心ESCRT-III成员HvSNF7a / CHMP4和HvVPS24 / CHMP3以及相关的ESCRT-III成分HvVPS60a / CHMP5用于大麦胚乳的瞬时定位研究。体内共聚焦显微镜分析表明，重组表达的HvSNF7a，HvVPS24和HvVPS60a的定位在大麦胚乳中有所不同。 HvSNF7a诱导大的团聚，而HvVPS24主要在糊粉和亚aleurone中显示胞质定位。相反，HvVPS60a强烈定位在糊粉层的质膜上。在亚aleurone中，在质膜和液泡膜上发现HvVPS60a的程度较小。这些结果表明，ESCRT-III的稳态关联可能受胚乳中细胞层特异性蛋白沉积或内膜系统运输和重塑的影响。我们显示人工单泛素化拟南芥质膜蛋白的排序被糊粉中的HvVPS60a抑制。讨论了HvVPS60a在不同细胞层特异性运输途径中的参与，这通过HvVPS60a在糊粉质膜和亚aleurone的PSV膜上的定位反映出来。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号