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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Xenopus LAP2 beta protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability
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Xenopus LAP2 beta protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

机译：爪蟾的LAP2 beta蛋白敲低影响lamin B和核孔蛋白的位置，并影响细胞核的组装和细胞活力

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摘要

Xenopus LAP2 beta protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2 beta fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with XLAP2 beta antibody. Knockdown of the XLAP2 beta protein expression in XTC cells by synthetic siRNA and plasmid encoded siRNA resulted in nuclear abnormalities including changes in shape of nuclei, abnormal chromatin structure, loss of nuclear envelope, mislocalization of integral membrane proteins of INM such as lamin B2, mislocalization of nucleoporins, and cell death. Based on timing of cell death, we suggest mechanism associated with nucleus reassembly or with entry into mitosis. This confirms that Xenopus LAP2 protein is essential for the maintenance of cell nucleus integrity and the process of its reassembly after mitosis.
机译：非洲爪蟾LAP2β蛋白是XTC细胞中表达的单一同工型。该蛋白质位于核包膜和细胞核内部的异染色质簇上。大部分XLAP2 beta片段在相间不与TPX2蛋白共定位，也不能与XLAP2 beta抗体免疫沉淀。通过合成siRNA和质粒编码的siRNA抑制XTC细胞中XLAP2β蛋白表达，导致核异常，包括核形状变化，染色质结构异常，核被膜丢失，INM整体膜蛋白（如层粘连蛋白B2）定位错误，定位错误和细胞死亡。根据细胞死亡的时间，我们建议与细胞核重组或进入有丝分裂有关的机制。这证实非洲爪蟾LAP2蛋白对于维持细胞核完整性及其在有丝分裂后的重组过程至关重要。

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