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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Biological potential of nanomaterials strongly depends on the suspension media: experimental data on the effects of fullerene C-60 on membranes
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Biological potential of nanomaterials strongly depends on the suspension media: experimental data on the effects of fullerene C-60 on membranes

机译：纳米材料的生物潜力在很大程度上取决于悬浮介质：关于富勒烯C-60对膜的影响的实验数据

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Fullerenes (C-60) are some of the most promising carbon nanomaterials to be used for medical applications as drug delivery agents. Computational and experimental studies have proposed their ability to enter cells by penetrating lipid bilayers. The aim of our study was to provide experimental evidence on whether pristine C-60 in physiological media could penetrate cell membranes. The effect was tested on phospholipid vesicles (liposomes) composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and validated on isolated human red blood cells (RBCs). We incubated the liposomes in an aqueous suspension of C-60 and dissolved the lipids and C-60 together in chloroform and subsequently formatted the liposomes. By differential scanning calorimetry measurements, we assessed the effect of C-60 on the phospholipid thermal profile. The latter was not affected after the incubation of liposomes in the C-60 suspension; also, a shape transformation of RBCs did not occur. Differently, by dispersing both C-60 and the phospholipids in chloroform, we confirmed the possible interaction of C-60 with the bilayer. We provide experimental data suggesting that the suspension medium is an important factor in determining the C-60-membrane interaction, which is not always included in computational studies. Since the primary particle size is not the only crucial parameter in C-60-membrane interactions, it is important to determine the most relevant characteristics of their effects on membranes.
机译：富勒烯（C-60）是一些最有前途的碳纳米材料，可用于医疗应用中作为药物输送剂。计算和实验研究提出了它们通过穿透脂质双层进入细胞的能力。我们研究的目的是提供有关生理培养基中原始C-60是否可以穿透细胞膜的实验证据。测试了对由1,2-二棕榈酰-sn-甘油-3-磷酸胆碱组成的磷脂囊泡（脂质体）的作用，并在分离的人红细胞（RBC）上进行了验证。我们将脂质体在C-60的水悬浮液中孵育，然后将脂质和C-60一起溶解在氯仿中，然后将脂质体格式化。通过差示扫描量热法测量，我们评估了C-60对磷脂热谱的影响。脂质体在C-60悬浮液中孵育后，后者不受影响;另外，没有发生红细胞的形状转变。不同的是，通过将C-60和磷脂分散在氯仿中，我们确认了C-60与双层的可能相互作用。我们提供的实验数据表明，悬浮介质是决定C-60膜相互作用的重要因素，而计算研究中并不总是如此。由于初级粒径不是C-60膜相互作用的唯一关键参数，因此重要的是确定其对膜的影响的最相关特征。

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