...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >CHANGES IN INTRACELLULAR PH DURING ZOOSPOROGENESIS IN PHYTOPHTHORA CINNAMOMI
【24h】

CHANGES IN INTRACELLULAR PH DURING ZOOSPOROGENESIS IN PHYTOPHTHORA CINNAMOMI

机译：褐藻致生菌过程中细胞内pH的变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Changes in intracellular pH (pH(i)) have been examined during zoosporogenesis in Phytophthora cinnamomi in order to elucidate the molecular mechanisms that bring about initiation of the orderly cleavage of the sporangium. By microinjecting a dextran-conjugated pH-sensitive fluorescent indicator, 2',7'-bis(2-carboxy ethyl)-5 (6)-carboxyfluorescein (BCECF dextran), and using fluorescence ratio image analysis, the pH(i) of a sporangium was monitored throughout the process of zoosporogenesis. The pH(i) of mature sporangia was found to remain constant at pH 6.84 +/- 0.05 (mean +/- SE, n = 6) at room temperature (24 degrees C), but increased to 7.04 +/- 0.04 (p < 0.001) during a 20 min cold treatment at 19 degrees C. As the process of zoosporogenesis proceeded, the increased level of pH(i) was maintained for 30-40 min after initiation of the cold shock. Zoosporogenesis was blocked when the pH(i) of the sporangium was held constant by microinjecting with 10 mM HEPES buffer (pH 7.0) before cytoplasmic cleavage was induced by a cold shock. In contrast, no inhibitory effect was observed when sporangia were microinjected with HEPES buffer after the cold shock. These results indicate that initiation of cytoplasmic cleavage requires a rise in pH(i). [References: 34]
机译：为了阐明引起孢子囊有序裂解的分子机制，在疫霉菌的游动发生过程中检查了细胞内pH（pH（i））的变化。通过微注射右旋糖酐共轭的pH敏感荧光指示剂2'，7'-双（2-羧基乙基）-5（6）-羧基荧光素（BCECF右旋糖酐），并使用荧光比图像分析，pH值孢子囊在整个动物孢子发生过程中都受到监测。发现成熟孢子囊的pH（i）在室温（24摄氏度）下保持恒定于pH 6.84 +/- 0.05（平均值+/- SE，n = 6），但增加到7.04 +/- 0.04（p在小于等于0.001）的温度下于19摄氏度进行20分钟的冷处理。随着游动孢子发生过程的进行，冷休克开始后，pH（i）的升高水平保持30-40分钟。当孢子囊的pH（i）保持恒定时，通过冷注射诱导胞质分裂之前，用10 mM HEPES缓冲液（pH 7.0）显微注射，可以阻止动物孢子发生。相反，冷休克后用HEPES缓冲液微量注射孢子囊没有观察到抑制作用。这些结果表明细胞质裂解的开始需要pH（i）的升高。 [参考：34]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号