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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >AN NMR MICROSCOPIC STUDY OF GRAPE (VITIS VINIFERA L)
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AN NMR MICROSCOPIC STUDY OF GRAPE (VITIS VINIFERA L)

机译：葡萄的葡萄核磁共振显微镜研究

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Mature healthy grape berries and berries wound-inoculated with the fungus Botrytis cinerea were examined by H-I NMR microimaging using 2D and 3D spin echo and gradient echo procedures. These NMR images were compared with representations obtained by conventional histology, where possible using the same specimens. 3D imaging datasets from excised seeds were reconstructed by surface rendering and maximum intensity projection to allow interpretation of their internal structure. T-2-weighted spin echo images revealed the major features of the pericarp, septum and loculi of whole berries. T-1-weighted images were less discriminatory of parenchyma tissues in the fruit but revealed the endosperm in seeds as a chemically shifted feature. A non-invasive study by T-1-weighted spin echo NMR imaging of infection by B. cinerea over a 6-day period showed that the disease spread throughout the exocarp but failed to spread in the mesocarp, a result confirmed by histological examination of the same specimen. Surface rendering of 3D datasets of excised seeds revealed the two ruminations of the endosperm and the distal location of the chalaza. The position of the embryonic axis was revealed in T-2-weighted maximum intensity projections. This noninvasive study revealed the need to apply a range of imaging techniques and parameters to visualise the structural features of the different parts of the grape berry. [References: 22]
机译：使用2D和3D自旋回波和梯度回波程序，通过1 H NMR显微成像检查成熟健康的葡萄浆果和用葡萄孢灰霉菌伤口接种的浆果。将这些NMR图像与通过常规组织学获得的表示进行比较，可能的话使用相同的样本。通过表面渲染和最大强度投影来重建来自切除种子的3D影像数据集，以解释其内部结构。 T-2加权自旋回波图像显示了整个浆果的果皮，中隔和位置的主要特征。 T-1加权图像对水果中薄壁组织的区分较少，但显示出种子中的胚乳是化学位移的特征。通过T-1加权自旋回波NMR成像对灰葡萄孢菌感染进行了为期6天的非侵入性研究，结果表明该病在整个外果皮中传播，但未能在中皮果皮中传播，这一结果通过组织学检查证实相同的标本。切下的种子的3D数据集的表面渲染显示了胚乳和chalaza的远端位置的两个反刍。胚胎轴的位置在T-2加权的最大强度投影图中显示。这项非侵入性研究表明，需要应用一系列成像技术和参数来形象化葡萄浆果不同部分的结构特征。 [参考：22]

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