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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >THE ROLD GENE FROM AGROBACTERIUM RHIZOGENES IS DEVELOPMENTALLY REGULATED IN TRANSGENIC TOBACCO
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THE ROLD GENE FROM AGROBACTERIUM RHIZOGENES IS DEVELOPMENTALLY REGULATED IN TRANSGENIC TOBACCO

机译:农杆菌根瘤菌的残基基因在转基因烟草中得到发育调控

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The rolD gene from Agrobacterium rhizogenes has recently been shown to induce striking precocity of flowering in transgenic tobacco. A transcriptional fusion between 578 bp of the rolD upstream regulating sequence and the GUS reporter gene has been transferred to tobacco plants and its expression analysed throughout the whole life cycle of the plant. A detailed histological analysis revealed that rolD is strongly expressed in elongating and differentiating tissues of each organ, from the mature embryo to the adult plant. In organs with determinate growth, such as cotyledons, leaves and floral parts, rolD expression follows the age gradient of the tissues. In mature tissues, rolD expression is high, begins to decrease with ageing and is switched off when senescence occurs. RolD is also active in the vascular system, in the procambium, pith- and receptacular-meristems. Our data show that expression of rolD is under developmental control as it correlates with, and may represent a molecular marker for, the elongation/expansion and maturation phases of the plant tissues. [References: 30]
机译:最近显示发根农杆菌的rolD基因在转基因烟草中诱导惊人的开花早熟。 rolD上游调节序列的578 bp与GUS报告基因之间的转录融合已转移至烟草植物,并在植物的整个生命周期中对其表达进行了分析。详细的组织学分析显示,rolD在从成熟胚胎到成年植物的每个器官的伸长和分化组织中强烈表达。在具有决定性生长的器官(例如子叶,叶子和花的部分)中,rolD表达遵循组织的年龄梯度。在成熟的组织中,rolD表达高,随着年龄的增长开始降低,并在发生衰老时被关闭。 RolD在血管系统,原位生,髓生和受体生血中也活跃。我们的数据表明rolD的表达处于发育控制之下,因为它与植物组织的延伸/扩展和成熟阶段相关,并可能代表其分子标记。 [参考:30]

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