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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >NET PROTON SECRETION AS A PARAMETER FOR NITRATE UPTAKE
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NET PROTON SECRETION AS A PARAMETER FOR NITRATE UPTAKE

机译：净质子分泌量作为硝化摄取的参数

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摘要

Nitrate uptake and its link to net proton secretion in wheat (Triticum aestivum L. cv. Tassilo, Caribo, and Astron) were investigated using a pH-stat system. Since nitrate is taken up in symport with protons, nitrate and proton fluxes should be correlated. Nitrate concentration in the medium, measured by HPLC, decreased in a linear manner. The addition of nitrate caused a drop in net proton secretion rate to negative values (net proton influx). Once nitrate concentration had been lowered to a well defined level, net proton secretion rate started to recover. This critical nitrate concentration depended on the initial nitrate concentration in the medium. A technique to derive nitrate uptake rates from time courses of net proton secretion was developed and is described. Briefly, this method requires the initial nitrate concentration and the time until the minimal net proton secretion rate is achieved. Results determined with this technique were found in excellent agreement to simultaneous direct measurements of nitrate uptake by HPLC. Measurement of net proton secretion therefore can be used as a parameter for nitrate uptake and as a screening method for uptake efficiency. This method was used to compare three varieties of a high nitrogen efficiency breeding line of wheat. The originally less nitrogen efficient variety outperformed the actually sold cultivar in nitrate uptake rate. [References: 21]
机译：使用pH调节系统研究了小麦（普通小麦Tassilo，Caribo和Astron）中硝酸盐的吸收及其与净质子分泌的关系。由于硝酸盐与质子共存，因此硝酸盐和质子通量应相互关联。通过HPLC测量，培养基中的硝酸盐浓度以线性方式降低。硝酸盐的添加导致净质子分泌速率下降至负值（净质子流入）。一旦硝酸盐浓度降低到确定的水平，净质子分泌率就开始恢复。该临界硝酸盐浓度取决于培养基中的初始硝酸盐浓度。开发并描述了一种从净质子分泌的时间过程中得出硝酸盐吸收率的技术。简而言之，该方法需要初始硝酸盐浓度和达到最小净质子分泌速率之前的时间。发现用该技术确定的结果与通过HPLC同时直接测量硝酸盐吸收非常一致。因此，净质子分泌的测量可用作硝酸盐吸收的参数和吸收效率的筛选方法。该方法用于比较小麦高氮效率育种系的三个品种。最初氮效率较低的品种在硝酸盐吸收率方面优于实际销售的品种。 [参考：21]

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