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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >MICROTUBULE ORGANIZATION DURING ZOOSPOROGENESIS IN ALLOMYCES MACROGYNUS
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MICROTUBULE ORGANIZATION DURING ZOOSPOROGENESIS IN ALLOMYCES MACROGYNUS

机译：雌雄同体异体造血过程中的微管组织

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摘要

The microtubule cytoskeleton and cytoplasmic organization of Allomyces macrogynus during zoosporogenesis was studied using light and electron microscopy. Indirect immunofluorescence methods revealed that the microtubule cytoskeleton progressed through three distinct stages of cytoplasmic distribution during zoospore development. During the first 10 minutes of zoosporogenesis, nuclei were strictly located in the periphery of the cytoplasm, and their associated centrosomes were positioned immediately adjacent to the plasma membrane. Microtubules emanated from centrosomes into the surrounding cytoplasm. Within 20 to 30 min after the induc tion of zoosporangial cleavage, nuclei migrated to new positions throughout the sporangial cytoplasm and microtubule arrays were primarily organized at and emanated from nuclear surfaces. During the final stage of zoosporogenesis, nuclear envelope-associated microtubules were not observed. Instead, primary organization of cytoplasmic microtubules returned to centrosomes (i.e., basal bodies) and flagella formation was evident The MPM-2 antibody, which recognizes phosphorylated epitopes of several proteins associated with microtubule nucleation, stained centrosome regions throughout zoosporogenesis but did not stain nuclear envelopes. [References: 44]
机译：利用光学和电子显微镜研究了大孢菌在游动孢子发生过程中的微管细胞骨架和胞质组织。间接免疫荧光法显示，在游动孢子发育过程中，微管细胞骨架经历了三个不同的胞质分布阶段。在游动孢子发生的前10分钟内，细胞核严格位于细胞质的外围，并且其相关的中心体紧邻质膜。微管从中心体散发到周围的细胞质中。在诱导动物孢子囊切割后20至30分钟内，核在整个孢子囊细胞质中迁移至新位置，并且微管阵列主要组织在核表面并从核表面发出。在游动孢子形成的最后阶段，未观察到核包膜相关的微管。取而代之的是，细胞质微管的主要组织返回到了中心体（即基体），鞭毛形成明显。MPM-2抗体可识别与微管成核有关的几种蛋白质的磷酸化表位，在整个动物孢子形成过程中都将中心体区域染色，但不染色核被膜。 [参考：44]

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