Immunological homologues of the Arabidopsis thaliana beta 1 tubulin are polyglutamylated in Nicotiana tabacum

Smertenko AP.; Hussey PJ.; Lawrence SL.

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Immunological homologues of the Arabidopsis thaliana beta 1 tubulin are polyglutamylated in Nicotiana tabacum

机译：拟南芥β1微管蛋白的免疫同源物在烟草中被聚谷氨酰化

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Peptide-specific antibody AABI, raised to the C-terminal 13 amino acids of Arabidopsis thaliana beta 1 tubulin, identifies a single electrophoretically separable beta-tubulin on 2-D-gel Western blots of total protein extracts from A. thaliana seedlings. We show that AABI crossreacts with two of the eight polyglutamylated beta-tubulin isoforms present in purified Nicotiana tabacum tubulin fractionated by high-resolution isoelectric focussing. Immunolocalisation studies using AAB1 revealed that the two N. tabacum polyglutamylated beta 1-tubulin isoforms are utilised in all four plant microtubule arrays (the interphase cortical array, the preprophase band, the spindle and the phragmoplast) indicating that there is no apparent subcellular sorting of these isotypes. [References: 34]

机译：拟南芥β1微管蛋白的C端13个氨基酸的肽特异性抗体AABI，在拟南芥幼苗总蛋白提取物的2-D凝胶Western印迹上鉴定了一个电泳可分离的β-微管蛋白。我们显示AABI与高分辨率的等电聚焦分离纯化的烟草烟草微管蛋白中存在的八个多聚谷氨酰β-微管蛋白同工型中的两个交叉反应。使用AAB1进行的免疫定位研究表明，在所有四个植物微管阵列（相间皮层阵列，前相带，纺锤体和原生质体）中均使用了两个烟草多谷氨酰胺化的β1-微管蛋白亚型，这表明没有明显的亚细胞分型。这些同种型。 [参考：34]

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《Protoplasma: An International Journal of Cell Biology》 |1998年第4期|共6页
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Smertenko AP.; Hussey PJ.; Lawrence SL.;
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中图分类细胞形态学;
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1. Immunological homologues of the Arabidopsis thaliana beta 1 tubulin are polyglutamylated in Nicotiana tabacum [J] . Smertenko AP., Hussey PJ., Lawrence SL. Protoplasma: An International Journal of Cell Biology . 1998,第3a4期

机译：拟南芥β1微管蛋白的免疫同源物在烟草中被聚谷氨酰化
2. AtRAB-H1b and AtRAB-H1c GTPases, homologues of the yeast Ypt6, target reporter proteins to the Golgi when expressed in Nicotiana tabacum and Arabidopsis thaliana [J] . Jorunn Nergaard Johansen1* Cheung-Ming Chow2† Ian Moore2 and Chris Hawes1‡ Journal of Experimental Botany . 2009,第11期

机译：酵母Ypt6的同源物AtRAB-H1 b 和AtRAB-H1 c GTP酶在烟草和拟南芥中表达时将报告蛋白靶向高尔基体。
3. Four flavonoid glycosyltransferases present in tea overexpressed in model plants Arabidopsis thaliana and Nicotiana tabacum for functional identification [J] . Xiaolan Jiang, Yufeng Shi, Xinlong Dai, Journal of chromatography, B. Analytical technologies in the biomedical and life sciences . 2018,第期

机译：在模型植物中存在的茶中的四种类黄酮糖基转移酶 rairalopsis thaliana 和尼古里亚纳Tabacum 用于功能识别
4. Using the chemical-inducible RNA interference (RNAi) system to elucidate the function(s) of glutamate receptor homologues in Arabidopsis thaliana [C] . P.A. Essah, L.-H. Liu, R.J. Davenport, International Plant Nutrition Colloquium . 2005

机译：使用化学诱导RNA干扰（RNAi）系统阐明拟南芥中谷氨酸受体同源物的功能
5. The 4-coumarate:coenzyme A ligases from Nicotiana tabacum and Arabidopsis thaliana: Characterization ofcDNA clones, gene families, recombinant proteins, and antisense transgenic plants [D] . Lee, Diana 1997

机译：烟草和拟南芥的4-香豆酸酯：辅酶A连接酶：cDNA克隆，基因家族，重组蛋白和反义转基因植物的鉴定
6. Transgenic Arabidopsis thaliana and Nicotiana tabacum overexpressing the Eucalyptus grandis Cellulose Synthase 3 and its expression pattern in different Eucalyptus species and tissues [O] . Wesley L Marques, Marcela Salazar, Eduardo Camargo, 2011

机译：过表达桉树纤维素合酶3的转基因拟南芥和烟草在不同种类和组织中的表达模式
7. Antifungal Activity of Recombinant Class V Chitinases from Nicotiana tabacum and Arabidopsis thaliana [O] . Takayuki Ohnuma, Toki Taira, Tamo Fukamizo 2012

机译：来自尼古利亚纳塔巴蟾蜍和拟南芥的重组级V Chitinase的抗真菌活性

Immunological homologues of the Arabidopsis thaliana beta 1 tubulin are polyglutamylated in Nicotiana tabacum

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