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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Oxidative burst and electrolyte leakage induced by sulfhydryl blockers and by membrane permeabilizing reagents in different organs of Egeria densa
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Oxidative burst and electrolyte leakage induced by sulfhydryl blockers and by membrane permeabilizing reagents in different organs of Egeria densa

机译：巯基阻断剂和膜透化剂在埃塞俄比亚不同器官中引起的氧化性破裂和电解质泄漏

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Sulfhydryl blockers, such as N-ethylmaleimide, iodoacetate and heavy metals induce a transitory stimulation of Or consumption and H2O2 production (oxidative burst) and a rapid release of electrolytes in leaves of various aquatic plants. The correlation between these two responses to N-ethylmaleimide or to Ag+ in separate organs and stages of leaf development was investigated in Egeria densa. Only adult leaves were able to respond to the sulfhydryl blockers with an oxidative burst, whereas this response was absent in immature growing leaves and in stem and root segments. In N-ethyl maleimide- as well as in Ag+-treated adult leaves the oxidative burst was constantly associated with a relevant electrolyte leakage. These data are consistent with a model in which the SH reagent would first interact with a plasmalemma protein, leading to an increase in passive permeability to ions and to the activation of an oxidative enzyme of the type of the superoxide synthase described for granulocytes. In its rum, active-oxygen species produced by the activated oxidase might further damage the plasma membrane, increasing its passive permeability. Digitonin and nystatin, two reagents known to cause a permeabilization of lipid membranes, induced in adult E. densa leaves a transient increase in the rate of O-2 consumption and H2O2 production and an electrolyte leakage very similar to those induced by sulfhydryl blockers. These effects, however, were nor influenced by the flavin analogues diphenylene iodonium and quinacrine, and were partially inhibited by the presence of CN- and salicylhydroxamic acid, thus suggesting the involvement of a different oxidase in the oxidative burst elicited by these reagents. [References: 33]
机译：巯基阻滞剂，例如N-乙基马来酰亚胺，碘乙酸盐和重金属，会短暂刺激Or消耗和H2O2产生（氧化爆发），并在各种水生植物叶片中迅速释放电解质。在Egeria densa中研究了这两种对N-乙基马来酰亚胺或Ag +在不同器官和叶片发育阶段的反应之间的相关性。只有成年的叶片能够对巯基阻滞剂产生氧化性爆发反应，而未成熟的叶片以及茎和根段则没有这种反应。在N-乙基马来酰亚胺以及经过Ag +处理的成年叶片中，氧化性爆裂一直与相关的电解液泄漏有关。这些数据与其中SH试剂首先与质膜蛋白相互作用，导致对离子的被动渗透性增加以及对粒细胞描述的超氧化物合酶类型的氧化酶活化的模型一致。在朗姆酒中，由活化的氧化酶产生的活性氧可能会进一步损害质膜，从而增加其被动渗透性。在成年大肠埃希氏菌中诱导产生的两种已知试剂-洋地黄素和制霉菌素，会导致脂质膜的通透性增加，从而使O-2消耗和H2O2的产生速率瞬时增加，电解质泄漏也与巯基阻滞剂诱导的非常相似。然而，这些作用不受黄素类似物二亚苯基碘鎓和奎那克林的影响，并且被CN-和水杨基异羟肟酸的存在部分抑制，因此表明这些试剂引起的氧化猝发中涉及不同的氧化酶。 [参考：33]

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