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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >CORTICAL MICROTUBULE INVOLVEMENT IN BORDERED PIT FORMATION IN SECONDARY XYLEM VESSEL ELEMENTS OF AESCULUS HIPPOCASTANUM L (HIPPOCASTANACEAE) - A CORRELATIVE STUDY USING ELECTRON MICROSCOPY AND INDIRECT IMMUNOFLUORESCENCE MICROSCOPY
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CORTICAL MICROTUBULE INVOLVEMENT IN BORDERED PIT FORMATION IN SECONDARY XYLEM VESSEL ELEMENTS OF AESCULUS HIPPOCASTANUM L (HIPPOCASTANACEAE) - A CORRELATIVE STUDY USING ELECTRON MICROSCOPY AND INDIRECT IMMUNOFLUORESCENCE MICROSCOPY

机译：刺五加（次生）的次生木质部元素中的边界坑形成中的微微管侵入-利用电子显微镜和间接免疫荧光显微镜进行的相关研究

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A correlative study, using indirect immunofluorescence microscopy (IIF) of anti-alpha-tubulin stained sections and transmission electron microscopy (TEM), gave details of the involvement of cortical microtubules (CMTs) in the development of bordered pits in secondary xylem vessel elements of Aesculus hippocastanum L. In addition, aspects of wall cytochemistry were studied during this process using the Thiery (PATAg) test, immunolocalization with the monoctonal antibodies JIM5 and JIM7, and a range of other cytochemical procedures. IIF showed that the alternately-arranged pits are pre-figured as perforations within a reticulum of randomly-oriented CMTs before any secondary wall thickening is obvious. Each incipient pit border is subsequently delimited by a circle of CMTs whose diameter decreases as deposition of secondary wall takes place around the perforation. These IIF observations are corroborated by a parallel TEM study. During the period of bordered pit formation, the secondary walls of the cell are lignifying. At maturity, however, the pit membrane is unlignified and continues to stain strongly with the monoclonal antibody JIM45, a marker of primary, ''juvenile'' wall. The results are discussed in terms of the relationship of the CMT cytoskeleton with development of bordered pits. [References: 69]
机译：一项相关研究使用抗α-微管蛋白染色的切片的间接免疫荧光显微镜（IIF）和透射电子显微镜（TEM），详细介绍了皮层微管（CMT）参与了次生木质部血管元件的有缘凹坑的形成。此外，在此过程中，使用Thiery（PATAg）测试，单链抗体JIM5和JIM7的免疫定位以及一系列其他细胞化学程序，研究了壁细胞化学的各个方面。 IIF显示，在任何次生壁明显增厚之前，在随机定向的CMT的网状区域内的孔眼中预先构筑了交替排列的凹坑。每个初始凹坑边界随后都由一圈CMT界定，这些CMT的直径随着穿孔周围次生壁的沉积而减小。平行TEM研究证实了这些IIF观察结果。在形成凹坑的期间，细胞的次生壁会木质化。然而，在成熟时，凹膜未被木质化，并继续被单克隆抗体JIM45（主要的“少年”壁的标记）强烈染色。根据CMT细胞骨架与边缘凹坑的发展之间的关系讨论了结果。 [参考：69]

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