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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Comparison of the redox activities in plasma membranes from roots and shoots of etiolated bean seedlings
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Comparison of the redox activities in plasma membranes from roots and shoots of etiolated bean seedlings

机译：黄化大豆幼苗根和茎上质膜氧化还原活性的比较

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Plasma membrane (PM) vesicles were purified in parallel from the roots and shoots of 6-day-old etiolated bean (Phaseolus vulgaris L,) seedlings, grown in water culture at 25 degrees C, by aqueous polymer tu two-phase partitioning. The purity of PM fractions was determine by measuring the activity of known marker enzymes (vanadate-sensitive Mg-ATPase, 1,3-beta-glycan synthase, latent ID-Pase, cytochrome c oxidase, and antimycin-A-insensitive cytochrome c reductase). NADH-(acceptor) oxidoreductase activities were determined with the following electron accepters: ferricyanide, cytochrorne c, duroquinone, monodehydroascorbate, Fe3+-EDTA, and oxygen. Cytochrome b content was also determined. In general, results show that redox activities are higher in the root PM than in the shoot PM which follows the glycan synthase II (PM marker) pattern. The relative activities of the distinct redox enzymes measured (activity profile) are remarkably similar in the root and shoot PM preparations. The cytochrome b content and level of ascorbate reduction were also imilar in both plant organs. However, the ratio of NADH-(acceptor) oxidoreductase activity to cptochrome content was significantly higher in roots when compared to the shoots. [References: 28]
机译：平行纯化膜，从6天龄的黄化豆（Phaseolus vulgaris L，）幼苗的根和芽中并行纯化，这些幼苗在25℃的水培养中通过水性聚合物进行两相分配。通过测量已知标记酶（钒酸盐敏感的Mg-ATPase，1,3-β-聚糖合酶，潜在的ID-Pase，细胞色素c氧化酶和抗霉素A不敏感的细胞色素c还原酶）的活性来确定PM组分的纯度。 ）。用以下电子受体确定NADH-（受体）氧化还原酶活性：铁氰化物，细胞色素c，二氢醌，一氢脱氢抗坏血酸酯，Fe3 + -EDTA和氧气。还测定了细胞色素b的含量。通常，结果表明，根部PM中的氧化还原活性高于茎部PM，后者遵循聚糖合酶II（PM标记）模式。在根部和茎部PM制剂中，所测得的不同氧化还原酶的相对活性（活性谱）非常相似。在两个植物器官中，细胞色素b的含量和抗坏血酸的减少水平也相似。然而，与芽相比，根中NADH-（受体）氧化还原酶活性与细胞色素含量的比率明显更高。 [参考：28]

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