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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Characterization of surface sugars on algal cells with fluorescein isothiocyanate-conjugated lectins
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Characterization of surface sugars on algal cells with fluorescein isothiocyanate-conjugated lectins

机译：荧光素异硫氰酸酯缀合的凝集素表征藻类细胞上的表面糖

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摘要

We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of cultured and environmental algae. Lectin-binding activity was markedly different between laboratory-cultured and environmental samples of the same species. Sugar composition of the cyanobacterium Anabaena cylindrica varied with growth cycle, although no clear pattern of change was observed. Akinetes typically showed lectin-binding activity higher than that of the vegetative cells or heterocysts throughout the growth cycle. Algae with mucilage showed greater lectin binding, indicating that mucilage contained more surface sugars accessible to the lectin probe compared with the cell wall surface. A low level of galactose and N-acetyl galactosamine (detected by peanut agglutinin) was associated with the surface mucilage of most algal species. Relatively high amounts of mannose, glucose, and N-acetyl glucosamine (detected by Concanavalin A, phytohaemagglutinin, and pokeweed mitogen) were also present. Lectin binding was shown to be a highly specific and sensitive approach to the examination of cell surface chemistry of both cultured and environmental algae and to the study of biodiversity in phytoplankton.
机译：我们使用荧光素异硫氰酸酯偶联的凝集素Concanavalin A，植物血凝素-赤藓凝集素，商陆有丝分裂原和花生凝集素的定性和定量荧光显微镜检查了许多培养和培养的细胞表面和细胞相关黏液的糖成分环保藻类。实验室培养的和相同物种的环境样品之间的凝集素结合活性明显不同。蓝藻鱼腥藻的糖组成随生长周期而变化，尽管未观察到明显的变化模式。在整个生长周期中，Akinetes通常显示出的凝集素结合活性要高于营养细胞或异胚囊的凝集素结合活性。带有黏液的藻类显示出更大的凝集素结合力，这表明与细胞壁表面相比，黏液包含更多的凝集素探针可及的表面糖。低水平的半乳糖和N-乙酰半乳糖胺（通过花生凝集素检测到）与大多数藻类的表面黏液有关。还存在相对较高的甘露糖，葡萄糖和N-乙酰氨基葡萄糖（通过伴刀豆球蛋白A，植物血凝素和商陆有丝分裂原检测到）。已证明凝集素结合是检查培养和环境藻类的细胞表面化学以及研究浮游植物生物多样性的高度特异性和灵敏的方法。

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