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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Effect of CO2 supply on formation of reactive oxygen species in Arabidopsis thaliana
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Effect of CO2 supply on formation of reactive oxygen species in Arabidopsis thaliana

机译：二氧化碳供应对拟南芥活性氧形成的影响

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摘要

Light-induced generation of reactive oxygen species (ROS) in 2-week-old leaves of Arabidopsis thaliana was studied by means of the ROS-sensitive dyes nitroblue tetrazolium (NBT) and 5-(and-6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCF-DA). Superposition of pictures of chlorophyll fluorescence and DCF fluorescence indicated that the origin of ROS was in the chloroplasts. Experiments were done with zero, 0.1, or 10 mM NaHCO3 in the infiltration medium. Energy quenching in photosystem II was higher under low CO2 concentrations as measured by chlorophyll fluorescence. DCF fluorescence showed that CO2 deficiency led to an increase of ROS generation. In contrast, the photosystem II inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea reduced the light-induced increase of DCF fluorescence. This indicates that ROS production does not primarily result from over-reduction of photosystem II as caused by impeding electron flow in the electron transfer chain. More likely, it is an effect of diverting electron flux normally aimed at carboxylation in the Calvin cycle to other sinks more prone to the generation of toxic radicals. There was no significant effect of salicyl hydroxamate ( a blocker of the alternative oxidase), showing that the mitochondrial electron transfer chain seems to play a minor role as already indicated by the superposition of chlorophyll and DCF fluorescence.
机译：利用ROS敏感染料硝基蓝四唑（NBT）和5-（和-6）-羧基-2'，研究了在2周大的拟南芥叶片中光诱导产生的活性氧（ROS）。 7'-二氯二氢荧光素二乙酸酯（DCF-DA）。叶绿素荧光和DCF荧光图像的叠加表明ROS的起源是叶绿体。在渗透介质中使用零，0.1或10 mM NaHCO3进行实验。通过叶绿素荧光测量，在低CO2浓度下，光系统II中的能量猝灭较高。 DCF荧光显示CO2缺乏导致ROS生成增加。相反，光系统II抑制剂3-（3,4-二氯苯基）-1,1-二甲基脲减少了光诱导的DCF荧光的增加。这表明ROS的产生主要不是由于光电子系统II的过度还原所致，因为光电子系统II的过度还原是由于阻碍电子转移链中的电子流动而引起的。这更有可能是将通常旨在加尔文循环中的羧化作用的电子通量转移到更易于产生毒性自由基的其他吸收剂的作用。水杨酸异羟肟酸酯（另一种氧化酶的阻滞剂）没有显著作用，表明线粒体电子转移链似乎起着次要作用，正如叶绿素和DCF荧光的叠加所表明的那样。

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