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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >New insights from MALDI-ToF MS, NMR, and GC-MS: mass spectrometry techniques applied to palynology
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New insights from MALDI-ToF MS, NMR, and GC-MS: mass spectrometry techniques applied to palynology

机译：MALDI-ToF MS，NMR和GC-MS的新见解：应用于孢粉学的质谱技术

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摘要

The present study for the first time describes the application of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-ToF MS) to palynology. With an accessible mass range of up to about 350,000 Da at subpicomolar range, this technique is ideal for the characterisation of bio-macromolecules, such as sporopollenin, found in fossil and extant pollen and spore walls, which often can only be isolated in very small quantities. At this stage, the limited solubility of sporopollenin allows for the identification of sections of this biopolymer, but with the optimisation of MALDI-ToF matrices, further structure elucidation will become possible. Furthermore, gas chromatography-mass spectrometry (GC-MS) and H-1 nuclear magnetic resonance (H-1 NMR) spectroscopy data obtained from a number of experiments revealed that some previously reported data were misinterpreted. These results add support to the hypothesis that common plasticizers were wrongly described as sporopollenin compounds.
机译：本研究首次描述了基质辅助激光解吸电离飞行时间质谱（MALDI-ToF MS）在孢粉学中的应用。该技术在亚皮摩尔范围内可达到的质量范围高达约350,000 Da，是表征生物大分子（例如孢粉）的理想方法，该分子存在于化石和现存的花粉壁和孢子壁中，通常只能在非常小的情况下进行分离数量。在这一阶段，孢粉的有限溶解度允许鉴定该生物聚合物的部分，但是随着MALDI-ToF基质的优化，进一步的结构阐明将成为可能。此外，从许多实验中获得的气相色谱-质谱（GC-MS）和H-1核磁共振（H-1 NMR）光谱数据表明，以前报道的某些数据被误解了。这些结果为以下假设提供了依据：常见的增塑剂被错误地描述为孢粉蛋白化合物。

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