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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Isolation and identification of antifungal N-butylbenzenesulphonamide produced by Pseudomonas sp AB2
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Isolation and identification of antifungal N-butylbenzenesulphonamide produced by Pseudomonas sp AB2

机译：假单胞菌sp AB2产生的抗真菌N-丁基苯磺酰胺的分离与鉴定

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摘要

An antifungal bacterial strain, isolated from a greenhouse soil sample, inhibits growth of microflora nearby. It was selected for further studies of bacterial antifungal properties. This isolate was identified as a Pseudomonas sp. based on carbohydrate utilization, and other biochemical and physiological tests. Petri plate assay revealed that the Pseudomonas sp. exhibited antifungal activity against the plant pathogens, Pythium ultimum, Rhizoctonia solani, Phytophthora capsici, Botrytis cinerea and Fusarium oxysporum. Using direct inhibition bioassay on TLC plates after ethyl acetate extraction of the culture filtrate, we correlated antifungal activity with production of antifungal compounds. An antifungal antibiotic was isolated from the culture filtrate and was identified as N-butylbenzenesulphonamide. ED50 values of the N-butylbenzenesulphonamide against P. ultimum, P. capsici, R. solani, and B. cinerea were 73, 41, 33 and 102 ppm, respectively. [References: 20]
机译：从温室土壤样本中分离出的一种抗真菌细菌菌株可抑制附近微生物群落的生长。选择它用于细菌抗真菌特性的进一步研究。该分离物被鉴定为假单胞菌属。基于碳水化合物利用以及其他生化和生理学测试。培养皿平板分析显示假单胞菌属。表现出对植物病原体，终生腐霉，茄根霉，辣椒疫霉，灰葡萄孢和尖孢镰刀菌的抗真菌活性。在乙酸乙酯提取培养物滤液后，在TLC板上使用直接抑制生物测定法，我们将抗真菌活性与抗真菌化合物的产生相关联。从培养物滤液中分离出一种抗真菌抗生素，并将其鉴定为N-丁基苯磺酰胺。 N-丁基苯磺酰胺对P.ultimum，P.capsici，R.solani和B.cinerea的ED50值分别为73、41、33和102 ppm。 [参考：20]

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