...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Antibiotics: An International Journal >Role of the AraC-XylS family regulator YdeO in multi-drug resistance of Escherichia coli.
【24h】

Role of the AraC-XylS family regulator YdeO in multi-drug resistance of Escherichia coli.

机译：AraC-XylS家族调节因子YdeO在大肠杆菌的多重耐药性中的作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Multi-drug efflux pumps contribute to the resistance of Escherichia coli to many antibiotics and biocides. In this study, we report that the AraC-XylS family regulator YdeO increases the multi-drug resistance of E. coli through activation of the MdtEF efflux pump. Screening of random fragments of genomic DNA for their ability to increase beta-lactam resistance led to the isolation of a plasmid containing ydeO, which codes for the regulator of acid resistance. When overexpressed, ydeO significantly increased the resistance of the E. coli strain to oxacillin, cloxacillin, nafcillin, erythromycin, rhodamine 6G and sodium dodecyl sulfate. The increase in drug resistance caused by ydeO overexpression was completely suppressed by deleting the multifunctional outer membrane channel gene tolC. TolC interacts with different drug efflux pumps. Quantitative real-time PCR showed that YdeO activated only mdtEF expression and none of the other drug efflux pumps in E. coli. Deletion of mdtEF completely suppressed the YdeO-mediated multi-drug resistance. YdeO enhances the MdtEF-dependent drug efflux activity in E. coli. Our results indicate that the YdeO regulator, in addition to its role in acid resistance, increases the multi-drug resistance of E. coli by activating the MdtEF multi-drug efflux pump.
机译：多药外排泵有助于大肠杆菌抵抗多种抗生素和杀生物剂。在这项研究中，我们报告说，AraC-XylS家族调节剂YdeO通过激活MdtEF外排泵来增加大肠杆菌的多重耐药性。筛选基因组DNA随机片段增加β-内酰胺抗性的能力导致分离出含有ydeO的质粒，该质粒编码耐酸性的调节剂。当过表达时，ydeO会显着增加大肠杆菌菌株对奥沙西林，氯沙西林，萘夫西林，红霉素，若丹明6G和十二烷基硫酸钠的抗性。通过删除多功能外膜通道基因tolC，完全抑制了ydeO过表达引起的耐药性增加。 TolC与不同的药物外排泵相互作用。实时定量PCR显示，YdeO仅激活mdtEF表达，而没有激活其他药物在大肠杆菌中的外排泵。删除mdtEF完全抑制了YdeO介导的多药耐药性。 YdeO增强了大肠杆菌中MdtEF依赖的药物外排活性。我们的结果表明，YdeO调节剂除了在耐酸性方面发挥作用外，还通过激活MdtEF多种药物外排泵提高了大肠杆菌的多种药物耐受性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号