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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Antimicrobial peptides from arachnid venoms and their microbicidal activity in the presence of commercial antibiotics
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Antimicrobial peptides from arachnid venoms and their microbicidal activity in the presence of commercial antibiotics

机译：蜘蛛毒液中的抗菌肽及其在商业抗生素存在下的杀微生物活性

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摘要

Two antimicrobial peptides (AMPs), named La47 and Css54, were isolated from the venom of the spider Lachesana sp. and from the scorpion Centruroides suffusus suffusus, respectively. The primary structures of both La47 and Css54 were determined using N-terminal sequencing and mass spectrometry. La47 is identical to the AMP latarcin 3a obtained previously from the venom of the spider Lachesana tarabaevi, but the primary structure of Css54 is unique having 60% identities to the AMP ponericin-W2 from the venom of the ant Pachycondyla goeldii. Both La47 and Css54 have typical α-helix secondary structures in hydrophobic mimicking environments. The biological activities of both La47 and Css54 were compared with the AMP Pin2 isolated from the venom of the scorpion Pandinus imperator. La47 has lower antimicrobial and hemolytic activities compared with Css54 and Pin2. In addition, La47 and Pin2 were evaluated in the presence of the commercial antibiotics, chloramphenicol, ampicillin, novobiocin, streptomycin and kanamycin. Interestingly, the best antimicrobial combinations were obtained with mixtures of La47 and Pin2 with the antibiotics chloramphenicol, streptomycin and kanamycin, respectively. Furthermore, the novel peptide Css54 was evaluated in the presence of antibiotics used for the treatment of tuberculosis, isoniazid, rifampicin, pyrazinamide and ethambutol. Although the mixtures of Css54 with isoniazid, pyrazinamide or ethambutol inhibit the growth of Staphylococcus aureus, the best effect was found with rifampicin. Overall, these data show a motivating outlook for potential clinical treatments of bacterial infections using AMPs and commercial antibiotics.
机译：从蜘蛛Lachesana sp的毒液中分离出了两种名为La47和Css54的抗菌肽（AMPs）。和分别来自蝎子Centruroides suffusus suffusus。 La47和Css54的一级结构均使用N端测序和质谱测定。 La47与先前从蜘蛛Lachesana tarabaevi的毒液中获得的AMP latarcin 3a相同，但Css54的一级结构独特，与来自蚂蚁Pachycondyla goeldii的毒液的AMP ponericin-W2具有60％的同一性。在疏水模拟环境中，La47和Css54均具有典型的α-螺旋二级结构。将La47和Css54的生物学活性与从蝎子Pandinus imperator毒液中分离出的AMP Pin2进行了比较。与Css54和Pin2相比，La47具有较低的抗菌和溶血活性。此外，在市售抗生素，氯霉素，氨苄青霉素，新生霉素，链霉素和卡那霉素的存在下对La47和Pin2进行了评估。有趣的是，使用La47和Pin2分别与抗生素氯霉素，链霉素和卡那霉素的混合物可获得最佳的抗菌组合。此外，在用于治疗结核病，异烟肼，利福平，吡嗪酰胺和乙胺丁醇的抗生素存在下评估了新型肽Css54。尽管Css54与异烟肼，吡嗪酰胺或乙胺丁醇的混合物可抑制金黄色葡萄球菌的生长，但利福平的效果最佳。总体而言，这些数据显示了使用AMP和商业抗生素对细菌感染进行潜在临床治疗的积极前景。

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