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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Chemical characterisation of disruptants of the Streptomyces coelicolor A3(2) actVI genes involved in actinorhodin biosynthesis
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Chemical characterisation of disruptants of the Streptomyces coelicolor A3(2) actVI genes involved in actinorhodin biosynthesis

机译：参与放线菌素生物合成的天蓝色链霉菌A3（2）actVI基因破坏基因的化学表征

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The actVI genetic region of Streptomyces coelicolor A3(2) is part of the biosynthetic gene cluster of actinorhodin (ACT), the act cluster, consisting of six ORFs: ORFB, ORFA, ORF1, ORF2, ORF3, ORF4. A newly devised method of ACT detection with a combination of HPLC and LC/MS was applied to the analysis of the disruptants of each ORE ACT was produced by those of ORFB, ORFA, ORF3, and ORF4. Instead of ACT, the ORF1 disruptant produced 3,8dihydroxy-1-methylanthraquinone-2-carboxylic acid (DMAC) and aloesaponarin II as shunt products. The ORF2 disruptant gave 4-dihydro-9-hydrsxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-acetic acid, (S)-DNPA. These results support our previous proposal for stereospecific pyran ring formation in the biosynthesis of ACT, most importantly suggesting that the actVI-ORF2 product would recognize (S)-DNPA as a substrate for stereospecific reduction at C-15. The disruptant of ORFA produced (S)-DNPA together with ACT, suggesting that actVI-ORFA might play a role such as stabilising the multicomponent, type II PKS complex. [References: 28]
机译：天蓝色链霉菌A3（2）的actVI遗传区域是肌动蛋白（ACT）的生物合成基因簇的一部分，该行为簇由六个ORF组成：ORFB，ORFA，ORF1，ORF2，ORF3，ORF4。一种新设计的结合HPLC和LC / MS的ACT检测方法被用于分析每个ORE ACT的破坏物，这些破坏物是由ORFB，ORFA，ORF3和ORF4产生的。代替ACT，ORF1破坏剂产生3,8dihydroxy-1-methylanthraquinone-2-羧酸（DMAC）和芦荟皂苷II作为分流产物。 ORF2破坏剂给出了4-dihydro-9-hydrsxy-1-methyl-10-oxo-3-H-naphtho- [2,3-c] -pyran-3-（S）-乙酸，（S）-DNPA 。这些结果支持了我们先前在ACT的生物合成中立体定向吡喃环形成的提议，最重要的是表明actVI-ORF2产品将（S）-DNPA识别为C-15立体定向还原的底物。 ORFA的破坏剂与ACT一起产生了（S）-DNPA，这表明actVI-ORFA可能起到稳定多组分II型PKS复合物的作用。 [参考：28]

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