...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Antibiotics: An International Journal >DNase I induced DNA degradation is inhibited by neomycin
【24h】

DNase I induced DNA degradation is inhibited by neomycin

机译：DNase I诱导的DNA降解被新霉素抑制

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Preparations of antimicrobials from biotechnological sources containing nucleic acids may serve as vector for the dissemination of resistance genes. An essential prerequisite for the acquisition of a new resistance phenotype in a transformational scenario is the availability of physically intact DNA molecules capable of transforming competent microorganisms. DNA is thought re, be an easy target for catabolic processes when present in the natural habitat of bacteria (eg. gastrointestinal tract, soil) due to the overall presence of nucleolytic enzymes. Aminoglycoside antibiotics are known to display a strong affinity to nucleic acids rendering these compounds to be primary candidates for exerting DNA protective functions in the gastrointestinal tract when applied orally during antibiotic chemotherapy. Using a DNase I protection assay it could be demonstrated that neomycin B at a concentration of 2 mM completely inhibited degradation of plasmid DNA in vitro. No inhibition of degradation was observed with streptomycin and kanamycin and the non-aminoglycoside antibiotics oxytetracycline and ampicillin under identical assay conditions. Thus, neomycin preparations may be able to promote structural integrity of contaminating DNA-fragments in DNase-rich environments. [References: 32]
机译：从含有核酸的生物技术来源制备的抗微生物剂可以用作传播抗性基因的载体。在转化方案中获得新抗性表型的必要前提是能够转化感受态微生物的物理完整DNA分子的可用性。由于脱氧核糖核酸酶的整体存在，DNA被认为在细菌的自然栖息地（例如胃肠道，土壤）中存在时容易成为分解代谢过程的靶标。已知氨基糖苷类抗生素对核酸具有很强的亲和力，从而使这些化合物成为在抗生素化学疗法中口服应用时在胃肠道中发挥DNA保护功能的主要候选药物。使用DNase I保护试验，可以证明浓度为2 mM的新霉素B在体外完全抑制了质粒DNA的降解。在相同的测定条件下，链霉素和卡那霉素以及非氨基糖苷类抗生素土霉素和氨苄青霉素均未观察到降解抑制作用。因此，在富含DNase的环境中，新霉素制剂可能能够促进污染性DNA片段的结构完整性。 [参考：32]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号